Behavior of variable V3 region from 16S rDNA of lactic acid bacteria in denaturing gradient gel electrophoresis

被引：96

作者：

Ercolini, D ^{[1
]}

Moschetti, G ^{[1
]}

Blaiotta, G ^{[1
]}

Coppola, S ^{[1
]}

机构：

[1] Univ Naples Federico II, Stn Microbiol Ind, Sez Microbiol Agr Alimentare Ambientale & Igiene, Dipartimento Sci Alimenti, I-80055 Portici, Italy

来源：

CURRENT MICROBIOLOGY | 2001年 / 42卷 / 03期

关键词：

D O I：

10.1007/s002840010204

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Separation of amplified V3 region from 16S rDNA by denaturing gradient gel electrophoresis (DGGE) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. Variable V3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera Pediococcus, Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, Weissella, and Streptococcus were analyzed. DGGE profiles obtained were species-specific for most of the cultures tested. Moreover, it was possible to group the remaining LAB reference strains according to the migration of their 16S V3 region in the denaturing gel. The results are discussed with reference to their potential in the analysis of LAB communities in food, besides shedding light on taxonomic aspects.

引用

页码：199 / 202

页数：4

共 21 条

[1]

Ampe F, 1999, APPL ENVIRON MICROB, V65, P5464

[2] Increased species diversity and extended habitat range of sulfur-oxidizing Thiomicrospira spp. [J].