Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid)

被引：7

作者：

Han, DW

Lee, MS

Park, BJ

Kim, JK

Park, JC

机构：

[1] Yonsei Univ, Coll Med, Dept Med Engn, Seoul 120752, South Korea

[2] Inje Univ, Dept Biomed Engn, Coll Biomed Sci & Engn, Kimhae 621749, South Korea

[3] Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 01期

关键词：

extracellular matrix; neurite outgrowth; poly(lactic-co-glycolic acid); rat neural cortical cell;

D O I：

10.1007/s10529-004-6585-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly( lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D-lysine ( PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for both neurofilament and MAP-2 with well-organized neurites and somatodendritic localization. The presence of PDL with LN or FN on the PLGA. films was essential for increased neural cell growth. Also, PLGA films coated with either PDL/LN or PDL/FN mixtures had higher neurite outgrowth and regular differentiation.

引用

页码：53 / 58

页数：6

共 20 条

[1] THE INFLUENCE OF FIBRONECTIN AND LAMININ DURING SCHWANN-CELL MIGRATION AND PERIPHERAL-NERVE REGENERATION THROUGH SILICON CHAMBERS [J].