Vertical transmission of bovine spongiform encephalopathy prions evaluated in a transgenic mouse model

被引：35

作者：

Castilla, J ^{[1
]}

Brun, A ^{[1
]}

Segundo, FDS ^{[1
]}

Salguero, FJ ^{[1
]}

Gutiérrez-Adán, A ^{[1
]}

Pintado, B ^{[1
]}

Ramírez, MA ^{[1
]}

del Riego, L ^{[1
]}

Torres, JM ^{[1
]}

机构：

[1] INIA, Ctr Invest & Sanidad Anim, Madrid 28130, Spain

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 13期

关键词：

D O I：

10.1128/JVI.79.13.8665-8668.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this work we show evidence of mother-to-offspring transmission in a transgenic mouse line expressing bovine PrP (boTg) experimentally infected by intracerebrall administration of bovine spongiform encephalopathy (BSE) prions. PrPres was detected in brains of newborns from infected mothers only when mating was allowed near to the clinical stage of disease, when brain PrPres deposition could be detected by Western blot analysis. Attempts to detect infectivity in milk after intracerebral inoculation in boTg mice were unsuccessful, suggesting the involvement of other tissues as carriers of prion dissemination. The results shown here prove the ability of BSE prions to spread centrifugally from the central nervous system to peripheral tissues and to offspring in a mouse model. Also, these results may complement previous epidemiological data supporting the occurrence of vertical BSE transmission in cattle.

引用

页码：8665 / 8668

页数：4

共 31 条

[1] PrPSc accumulation in placentas of ewes exposed to natural scrapie:: influence of foetal PrP genotype and effect on ewe-to-lamb transmission [J].