Repair in Escherichia coli alkB mutants of abasic sites and 3-methyladenine residues in DNA

被引:32
作者
Dinglay, S
Gold, B
Sedgwick, B [1 ]
机构
[1] Imperial Canc Res Fund, Clare Hall Labs, S Mimms EN6 3LD, Herts, England
[2] Univ Nebraska, Med Ctr, Eppley Inst Res Canc & Allied Dis, Omaha, NE 68198 USA
来源
MUTATION RESEARCH-DNA REPAIR | 1998年 / 407卷 / 02期
关键词
Escherichia coli alkB mutant; DNA methylation;
D O I
10.1016/S0921-8777(97)00065-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Escherichia coli alkB mutants an sensitive to methyl methanesulfonate and dimethylsulphate, and are defective in the processing of methylated DNA, The function of the AlkB protein has not been determined, Here we show that alkB mutants are not defective in repairing several different types of potentially toxic DNA lesions that are known to be generated by MMS, including apyrimidinic and apurinic sites, and secondary lesions that could arise at these sites (DNA-protein cross-links and DNA interstrand cross-links). Also, alkB mutants were not sensitive to MeOSO2-(CH2)(2)-Lex, a compound that alkylates the minor groove of DNA generating primarily 3-methyladenine. (C) 1998 Elsevier Science B.V.
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页码:109 / 116
页数:8
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