Online concentration of aristolochic acid I and II in Chinese medicine preparations by micellar electrokinetic chromatography

被引：24

作者：

Shi, Shenhua

Li, Wei

Liao, Yiping

Cai, Zongwei

Liu, Huwei ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem,Minist Educ, Key Lab Bioorg Chem & Mol Engn,Beijing Natl Lab M, Beijing 100871, Peoples R China

[2] Hong Kong Baptist Univ, Dept Chem, Kowloon, Hong Kong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1167卷 / 01期

基金：

中国国家自然科学基金;

关键词：

aristolochic acid; Chinese herbs nephropathy; online concentration; Chinese medicine preparations; field-enhanced sample injection; reversed electrode; polarity stacking mode;

D O I：

10.1016/j.chroma.2007.08.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In this study, an online concentration method in micellar electrokinetic chromatography (MEKC) applying field-enhanced sample injection (FESI) mode was developed for the detection of aristolochic acids (AAs) in Chinese medicine preparations. AA-I and AA-II were baseline separated with high separation efficiency, and 100-fold enhancement of the detection sensitivity was achieved compared with those obtained from normal capillary zone electrophoresis (CZE) or simple MEKC method. The proposed method was successfully applied for the determination of AAs in Chinese medicine preparations. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：120 / 124

页数：5

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