Capillary liquid chromatographic and automated MALDI-TOF mass spectrometric analysis of complex carbohydrate mixtures

被引：10

作者：

Grimm, R

Birrell, H

Ross, G

Charlwood, J

Camilleri, P

机构：

[1] SmithKline Beecham Pharmaceut, Harlow CM19 5AW, Essex, England

[2] Hewlett Packard Co, Chem Anal Grp Europe, D-76337 Waldbronn, Germany

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 18期

关键词：

D O I：

10.1021/ac980235h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Reproducible microbore reversed-phase HPLC methodology has been developed, suitable for the analysis of carbohydrates derivatized with 2-aminoacridone. The high extinction coefficient of this label at around 260 nm, combined with the use of a new diode array microflow cell, has allowed detection at femtomole levels. Analytes were also robotically collected onto MALDI targets, and mass spectrometric information was acquired for carbohydrate present at low picomole levels. This technology was successfully applied to mixtures of linear and branched oligosaccharides, including sialylated species.

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收藏

页码：3840 / 3844

页数：5

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