A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel

被引：28

作者：

Wu, TP ^{[1
]}

Ruan, KC ^{[1
]}

Liu, WY ^{[1
]}

机构：

[1] ACAD SINICA, SHANGHAI INST BIOCHEM, SHANGHAI 200031, PEOPLES R CHINA

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 17期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1093/nar/24.17.3472

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A practical fluorescence-labeling method for sequencing small RNAs by the traditional 'direct read out' on polyacrylamide gel electrophoresis was established. The 3' terminus of RNA was oxidized into dialdehyde by sodium periodate and then labeled with fluorescein-5-thiosemicarbazide through the condensation reaction between carbazide and aldehyde. The fluorescence-labeled RNA was partially degraded enzymatically and fractionated by polyacrylamide gel electrophoresis. The fluorescent bands were visualised by ultraviolet photography. A partial sequence of yeast 5S rRNA was determined. The result indicates that this method can be used in sequencing small RNAs rapidly, conveniently and safely.

引用

页码：3472 / 3473

页数：2

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