Transmission of tobacco rattle virus isolate PpK20 by its nematode vector requires one of the two non-structural genes in the viral RNA 2

被引：41

作者：

Hernandez, C

Visser, PB

Brown, DJF

Bol, JF

机构：

[1] GORLAEUS LABS,INST MOL PLANT SCI,NL-2300 RA LEIDEN,NETHERLANDS

[2] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1997年 / 78卷

关键词：

D O I：

10.1099/0022-1317-78-2-465

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tobacco rattle virus isolate PpK20 is transmitted by the nematode Paratrichodorus pachydermus. RNA 2 of the virus determines vector transmissibility and encodes the viral coat protein and two non-structural proteins with molecular masses of 29 . 4 kDa and 32 . 8 kDa. Deletions and a frameshift in the two non-structural genes did not interfere with encapsidation or co-replication of RNA 2 with RNA 1 after mechanical inoculation of plants. Mutations that affected the 29 . 4K gene or both non-structural genes abolished nematode transmission, whereas a large deletion in the 32 . 8K gene had no effect on transmission by P. pachydermus. It is concluded that the 29 . 4K gene but not the 32 . 8K gene is involved in transmission of isolate PpK20 by this vector.

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页码：465 / 467

页数：3

相关论文

共 13 条

[1] RNA-2 OF TOBACCO RATTLE VIRUS-STRAIN TCM ENCODES AN UNEXPECTED GENE [J].

ANGENENT, GC ;

LINTHORST, HJM ;

VANBELKUM, AF ;

CORNELISSEN, BJC ;

BOL, JF .

NUCLEIC ACIDS RESEARCH, 1986, 14 (11) :4673-4682

[2]

Brown D.J.F., 1989, Bulletin OEPP, V19, P611

[3] TRANSMISSION OF VIRUSES BY PLANT NEMATODES [J].

BROWN, DJF ;

ROBERTSON, WM ;

TRUDGILL, DL .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 1995, 33 :223-249

[4] THE COMPLETE NUCLEOTIDE-SEQUENCE OF PEBV-RNA2 REVEALS THE PRESENCE OF A NOVEL OPEN READING FRAME AND PROVIDES INSIGHTS INTO THE STRUCTURE OF TOBRAVIRAL SUBGENOMIC PROMOTERS [J].

GOULDEN, MG ;

LOMONOSSOFF, GP ;

DAVIES, JW ;

WOOD, KR .

NUCLEIC ACIDS RESEARCH, 1990, 18 (15) :4507-4512

[5] THE COMPLETE NUCLEOTIDE-SEQUENCE OF TOBACCO RATTLE VIRUS RNA-1 [J].

HAMILTON, WDO ;

BOCCARA, M ;

ROBINSON, DJ ;

BAULCOMBE, DC .

JOURNAL OF GENERAL VIROLOGY, 1987, 68 :2563-2575

[6] Serial passage of tobacco rattle virus under different selection conditions results in deletion of structural and nonstructural genes in RNA 2 [J].

Hernandez, C ;

Carette, JE ;

Brown, DJF ;

Bol, JF .

JOURNAL OF VIROLOGY, 1996, 70 (08) :4933-4940

[7] SEQUENCE OF RNA-2 OF A NEMATODE-TRANSMISSIBLE ISOLATE OF TOBACCO RATTLE VIRUS [J].

HERNANDEZ, C ;

MATHIS, A ;

BROWN, DJF ;

BOL, JF .

JOURNAL OF GENERAL VIROLOGY, 1995, 76 :2847-2851

[8] Multiple virus genes involved in the nematode transmission of pea early browning virus [J].

MacFarlane, SA ;

Wallis, CV ;

Brown, DJF .

VIROLOGY, 1996, 219 (02) :417-422

[9] SEQUENCE COMPARISON OF RNA2 OF NEMATODE-TRANSMISSIBLE AND NEMATODE-NON-TRANSMISSIBLE ISOLATES OF PEA EARLY-BROWNING VIRUS SUGGESTS THAT THE GENE ENCODING THE 29-KDA PROTEIN MAY BE INVOLVED IN NEMATODE TRANSMISSION [J].

MACFARLANE, SA ;

BROWN, DJF .

JOURNAL OF GENERAL VIROLOGY, 1995, 76 :1299-1304

[10] PEA EARLY BROWNING VIRUS RNA1 ENCODES 4 POLYPEPTIDES INCLUDING A PUTATIVE ZINC-FINGER PROTEIN [J].

MACFARLANE, SA ;

TAYLOR, SC ;

KING, DI ;

HUGHES, G ;

DAVIES, JW .

NUCLEIC ACIDS RESEARCH, 1989, 17 (06) :2245-2260