The determination of the extracellular concentration of brain glutamate using quantitative microdialysis

被引：59

作者：

Miele, M ^{[1
]}

Berners, M ^{[1
]}

Boutelle, MG ^{[1
]}

Kusakabe, H ^{[1
]}

Fillenz, M ^{[1
]}

机构：

[1] UNIV OXFORD,PHYSIOL LAB,OXFORD OX1 3PT,ENGLAND

来源：

BRAIN RESEARCH | 1996年 / 707卷 / 01期

关键词：

quantitative microdialysis; glutamate; rat striatum; enzyme assay; extracellular concentration; unanesthetized;

D O I：

10.1016/0006-8993(95)01371-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Quantitative microdialysis with two enzyme-based assays was used to determine the extracellular concentration of glutamate in the striatum of freely moving rats. From the difference between infused and dialysate glutamate a value of 3.0 +/- 0.6 mu M for the extracellular glutamate concentration was computed by regression analysis. The in vivo recovery, derived from the slope of the regression line, was 50%.

引用

页码：131 / 133

页数：3

共 14 条

[1] ONLINE MEASUREMENT OF BRAIN GLUTAMATE WITH AN ENZYME/POLYMER-COATED TUBULAR ELECTRODE [J].

BERNERS, MOM ;

BOUTELLE, MG ;

FILLENZ, M .

ANALYTICAL CHEMISTRY, 1994, 66 (13) :2017-2021

[2] ENZYME PACKED-BED SYSTEM FOR THE ONLINE MEASUREMENT OF GLUCOSE, GLUTAMATE, AND LACTATE IN BRAIN MICRODIALYSATE [J].

BOUTELLE, MG ;

FELLOWS, LK ;

COOK, C .

ANALYTICAL CHEMISTRY, 1992, 64 (17) :1790-1794

[3]

FILLENZ M, IN PRESS BEHAV BRAIN

[4]

HOLMANN M, 1994, ANNU REV NEUROSCI, V17, P31

[5] MASS-TRANSFER IN BRAIN DIALYSIS DEVICES - A NEW METHOD FOR THE ESTIMATION OF EXTRACELLULAR AMINO-ACIDS CONCENTRATION [J].

JACOBSON, I ;

SANDBERG, M ;

HAMBERGER, A .

JOURNAL OF NEUROSCIENCE METHODS, 1985, 15 (03) :263-268

[6] VERATRIDINE-INDUCED RELEASE INVIVO AND INVITRO OF AMINO-ACIDS IN THE RABBIT OLFACTORY-BULB [J].

JACOBSON, I ;

HAMBERGER, A .

BRAIN RESEARCH, 1984, 299 (01) :103-112

[7] GLUTAMATE RECEPTORS IN THE CENTRAL-NERVOUS-SYSTEM [J].

JONAS, P .

MOLECULAR BASIS OF ION CHANNELS AND RECEPTORS INVOLVED IN NERVE EXCITATION, SYNAPTIC TRANSMISSION AND MUSCLE CONTRACTION: IN MEMORY OF PROFESSOR SHOSAKU NUMA, 1993, 707 :126-135

[8] PURIFICATION AND PROPERTIES OF A NEW ENZYME, L-GLUTAMATE OXIDASE, FROM STREPTOMYCES SP X-119-6 GROWN ON WHEAT BRAN [J].