Expression of interferon-alpha receptor mRNA in the liver in chronic liver diseases associated with hepatitis C virus: Relation to effectiveness of interferon therapy

被引:21
作者
Fukuda, R
Ishimura, N
Ishihara, S
Tokuda, A
Satoh, S
Sakai, S
Akagi, S
Watanabe, M
Fukumoto, S
机构
[1] Department of Internal Medicine, Shimane Medical University, Izumo-shi, Shimane 693
关键词
Chronic hepatitis C; interferon-alpha receptor mRNA; interferon therapy;
D O I
10.1007/BF02358606
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
To investigate whether interferon-alpha receptor (IFN-alpha Rc) expression was related to the ef fectiveness of interferon therapy in hepatitis C virus (HCV)-associated chronic liver disease (CLD), IFN-alpha Rc mRNA was investigated by reverse transcription polymerase chain reaction (RT-PCR) in liver biopsies and peripheral blood mononuclear cells (PBMCs) from 40 patients with HCV-associated CLD who subsequently received IFN-alpha therapy. IFN-alpha Rc mRNA in the liver was detected in 18 of 20 (90%) responders to IFN and in 5 of 20 (25%) non-responders (P < 0.01). In PBMCs, IFN-alpha Rc mRNA was detected in all patients regardless of response to IFN. Increased histological hepatitis activity and liver fibrosis were significantly related to the absence of IFN-alpha Rc mRNA. The HCV-RNA genotype showed no significant relationship to IFN-alpha Rc mRNA expression. Our results suggest that IFN-alpha Rc mRNA expression in the liver, but not in PBMCs, is closely associated with the effectiveness of IFN-alpha therapy in HCV-associated CLD.
引用
收藏
页码:806 / 811
页数:6
相关论文
共 37 条
[1]   2-5A SYNTHETASE-ACTIVITY DOES NOT INCREASE IN INTERFERON-RESISTANT FRIEND-LEUKEMIA CELL VARIANTS TREATED WITH ALPHA-BETA-INTERFERON DESPITE THE PRESENCE OF HIGH-AFFINITY INTERFERON RECEPTOR-SITES [J].
AFFABRIS, E ;
ROMEO, G ;
BELARDELLI, F ;
JEMMA, C ;
MECHTI, N ;
GRESSER, I ;
ROSSI, GB .
VIROLOGY, 1983, 125 (02) :508-512
[2]   VARIOUS HUMAN INTERFERON ALPHA-SUBCLASSES CROSS-REACT WITH COMMON RECEPTORS - THEIR BINDING AFFINITIES CORRELATE WITH THEIR SPECIFIC BIOLOGICAL-ACTIVITIES [J].
AGUET, M ;
GROBKE, M ;
DREIDING, P .
VIROLOGY, 1984, 132 (01) :211-216
[3]  
AUGUET M, 1981, VIROLOGY, V114, P585
[4]  
BRANCA AA, 1982, J BIOL CHEM, V257, P13197
[5]  
DEGROOTE J, 1968, LANCET, V2, P626
[6]  
DEMAEYER E, 1988, INTERFERONS OTHER RE, P221
[7]  
DEMAEYER E, 1988, INTERFERON OTHER REV, P115
[8]  
DEMAEYER E, 1988, INTERFERONS OTHER RE, P91
[9]   Mutations in the nonstructural protein 5A gene and response to interferon in patients with chronic hepatitis C virus 1b infection [J].
Enomoto, N ;
Sakuma, I ;
Asahina, Y ;
Kurosaki, M ;
Murakami, T ;
Yamamoto, C ;
Ogura, Y ;
Izumi, N ;
Marumo, F ;
Sato, C .
NEW ENGLAND JOURNAL OF MEDICINE, 1996, 334 (02) :77-81
[10]   KINETICS OF INTERNALIZATION AND DEGRADATION OF SURFACE-BOUND INTERFERON IN HUMAN-LYMPHOBLASTOID CELLS [J].
EVANS, T ;
SECHER, D .
EMBO JOURNAL, 1984, 3 (12) :2975-2978