Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes

被引：7

作者：

Khrebtukova, I

Michaud, EJ

Foster, CM

Stark, KL

Garfinkel, DJ

Woychik, RP

机构：

[1] Case Western Reserve Univ, Rainbow Babies & Childrens Hosp, Dept Pediat, Cleveland, OH 44106 USA

[2] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN 37831 USA

[3] NCI, Frederick Canc Res & Dev Ctr, ABL Basic Res Program, Gene Regulat & Chromosome Biol Lab, Frederick, MD 21702 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1998年 / 401卷 / 1-2期

关键词：

microhomologous recombination; yeast; targeting construct; targeted mutagenesis; mouse mutation; functional genomics;

D O I：

10.1016/S0027-5107(98)00053-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a new procedure utilizing microhomologous recombination in yeast to generate targeting constructs for producing targeted mutations in mice. This procedure is rapid and efficient, and should be directly applicable to all mammalian genes. Moreover, only minimal information about the locus being targeted is required. The feasibility of this approach was demonstrated by producing another allele of the mouse Tg737 polycystic kidney gene. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：11 / 25

页数：15

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