Selective fluorescence detection of nitroxyl over nitric oxide in buffered aqueous solution using a conjugated metallopolymer

被引：38

作者：

Tennyson, Andrew G. ^{[1
]}

Do, Loi ^{[1
]}

Smith, Rhett C. ^{[1
]}

Lippard, Stephen J. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

POLYHEDRON | 2007年 / 26卷 / 16期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

poly(p-phenyleneethynylene); bithiophene; conjugated metallopolymer; fluorescent sensing; nitric oxide; nitroxyl;

D O I：

10.1016/j.poly.2007.04.003

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

A bithiophene-substituted poly(p-phenyleneethynylene) derivative (CP1) having water-solubilizing side chains was prepared and characterized. Copper(Il)-induced quenching of CP1 emission was quantified in H2O, MeCN/H2O (90:10), and pH 7.4, 50 mM HEPES, 100 mM KG buffer. In buffer, treatment of CPI-Cu(II) with nitroxyl (HNO) produces an immediate 2.1-fold increase in emission, whereas exposure to NO(g) effects no fluorescence restoration. The ability to distinguish HNO from NO chemically at physiological pH represents a productive step towards the development of selective, fluorescence-based biosensors for HNO. (c) 2007 Elsevier Ltd. All rights reserved.

引用

收藏

页码：4625 / 4630

页数：6

相关论文

共 46 条

[1] MOLECULAR ASSEMBLY OF PROTEINS AND CONJUGATED POLYMERS - TOWARD DEVELOPMENT OF BIOSENSORS [J].

AYYAGARI, MS ;

PANDE, R ;

KAMTEKAR, S ;

GAO, H ;

MARX, KA ;

KUMAR, J ;

TRIPATHY, SK ;

AKKARA, JA ;

KAPLAN, DL .

BIOTECHNOLOGY AND BIOENGINEERING, 1995, 45 (02) :116-121

[2] The application of factor analysis to solvatochromic parameters and pHs values for the standardization of potentiometric sensors in mobile phases used in liquid chromatography [J].

Barbosa, J ;

Marqués, I ;

Barrón, D ;

Sanz-Nebot, V .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1999, 18 (08) :543-549

[3] The reduction potential of nitric oxide (NO) and its importance to NO biochemistry [J].

Bartberger, MD ;

Liu, W ;

Ford, E ;

Miranda, KM ;

Switzer, C ;

Fukuto, JM ;

Farmer, PJ ;

Wink, DA ;

Houk, KN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :10958-10963

[4]

Buey J, 2000, ANGEW CHEM INT EDIT, V39, P608, DOI 10.1002/(SICI)1521-3773(20000204)39:3<608::AID-ANIE608>3.0.CO

[5]

2-W

[6] SULFUR-COORDINATED THIOPHENE AND DIBENZOTHIOPHENE IN CP'(CO)2RE(THIOPHENE) COMPLEXES [J].

CHOI, MG ;

ANGELICI, RJ .

ORGANOMETALLICS, 1991, 10 (07) :2436-2442

[7] PROPERTIES OF BASES IN ACETONITRILE AS SOLVENT .4. PROTON ACCEPTOR POWER AND HOMOCONJUGATION OF MONO- AND DIAMINES [J].

COETZEE, JF ;

PADMANAB.GR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1965, 87 (22) :5005-&

[8] Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species [J].

Crow, JP .

NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 1997, 1 (02) :145-157

[9] Luminescent properties of water-soluble conjugated metallopolymers and their application to fluorescent nitric oxide detection [J].

Do, Loi ;

Smith, Rhett C. ;

Tennyson, Andrew G. ;

Lippard, Stephen J. .

INORGANIC CHEMISTRY, 2006, 45 (22) :8998-9005

[10] Regioregular poly(thiophene-3-alkanoic acid)s:: water soluble conducting polymers suitable for chromatic chemosensing in solution and soild state [J].

Ewbank, PC ;

Loewe, RS ;

Zhai, L ;

Reddinger, J ;

Sauvé, G ;

McCullough, RD .

TETRAHEDRON, 2004, 60 (49) :11269-11275

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