The first fluorogenic assay for detecting a Baeyer-Villigerase activity in microbial cells

被引：30

作者：

Gutiérrez, MC ^{[1
]}

Sleegers, A ^{[1
]}

Simpson, HD ^{[1
]}

Alphand, V ^{[1
]}

Furstoss, R ^{[1
]}

机构：

[1] Univ Mediterranee, CNRS, Fac Sci Luminy, UMR 6111,Grp Biocatalyse & Chim Fine, F-13288 Marseille 9, France

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2003年 / 1卷 / 20期

关键词：

D O I：

10.1039/b306687h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The first fluorogenic assay allowing for detection of microbial enzymes able to perform Baeyer-Villiger oxidation is described. This is based on the use of 4-oxopentyl umbelliferyl ether I as a fluorogenic substrate. When Baeyer-Villigerases active against this test ketone are present in the selected whole cells, 1 is transformed into 3-hydroxypropyl umbelliferyl ether 3, which, in a subsequent step, releases the fluorescent product umbelliferone. Different microorganisms, known to be endowed with Baeyer-Villigerase activity, were assayed.

引用

页码：3500 / 3506

页数：7

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