Phenytoin and phenobarbital inhibit human HERG potassium channels

被引:83
作者
Danielsson, BR
Lansdell, K
Patmore, L
Tomson, T [1 ]
机构
[1] Karolinska Hosp & Inst, Dept Clin Neurosci, Div Neurol, S-17176 Stockholm, Sweden
[2] Uppsala Univ, Dept Pharmaceut Biosci, Div Toxicol, S-75124 Uppsala, Sweden
[3] Heriot Watt Univ, Edinburgh EH14 4AP, Midlothian, Scotland
关键词
phenytoin; phenobarbital; carbamazepine; HERG; I-Kr; sudden death;
D O I
10.1016/S0920-1211(03)00119-0
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Drugs that inhibit the cardiac rapid delayed rectifier potassium ion current (I-Kr) channel can be proarrhythmic and their clinical use has been associated with sudden unexpected death (SUD). Since SUD is about 20 times more common among people with epilepsy than in the general population. and Some data indicate that drug treatment may contribute. we tested the hypothesis that the classic antiepileptic drugs phenytoin (PHT). carbamazepine (CBZ), and phenobarbital (PB) have et potential to block I-Kr. The whole cell patch-clamp recording technique was used to Study the effects on I-Kr channels expressed by the human ether-a-go-go related gene (HERG) stably expressed in Human Embryo Kidney (HEK) 293 cells. Tail currents, which are purely related to HERG, were blocked with an IC50 (the concentration when 50% inhibition was obtained compared to control values) of 240 muM for PHT and 3 mM for PB. A 20% inhibition of tail currents was obtained at CBZ concentrations of 250 and 500 muM. Collective data Show that drugs with the same margins (ratio HERG IC50/unbound therapeutic concentration). as PHT and PB. may have arrhythmogenic potential. especially when used in predisposed patients;and in the case of drug-drug interactions. SUD in epilepsy is generally a Seizure-related phenomenon. However, our data Suggest that PI-IT and PB may play a contributing role, perhaps by making some patients more vulnerable to the cardiovascular depression induced by seizures. (C) 2003 Published by Elsevier B.V.
引用
收藏
页码:147 / 157
页数:11
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