Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria

被引：31

作者：

Chapuis, MP

Loiseau, A

Michalakis, Y

Lecoq, M

Estoup, A

机构：

[1] INRA, Ctr Biol & Gestion Populat, F-34988 Montferrier Sur Lez, Lez, France

[2] Ctr Etud Polymorphisme Microorganismes, CNRS, UMR 9926, IRD, Montpellier 5, France

[3] Ctr Cooperat Int Rech Agronom Dev, F-34398 Montpellier, France

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期

关键词：

locust; Locusta migratoria; microsatellites; null alleles; Orthoptera; pest;

D O I：

10.1111/j.1471-8286.2005.00987.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerise chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species.

引用

页码：554 / 557

页数：4

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Doyle J. L., 1987, Phytochemical Bulletin, V19, P11, DOI 10.2307/4119796

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