Analysis of microbial communities developed on the fouling layers of a membrane-coupled anaerobic bioreactor applied to wastewater treatment

被引：119

作者：

Calderon, Kadiya ^{[2
]}

Rodelas, Belen ^{[1
]}

Cabirol, Nathalie ^{[2
]}

Gonzalez-Lopez, Jesus

Noyola, Adalberto ^{[2
]}

机构：

[1] Univ Granada, Dept Microbiol, Fac Farm, Grp Microbiol Ambiental, E-18071 Granada, Spain

[2] Univ Nacl Autonoma Mexico, Inst Ingn, Grp Invest Proc Anaerobios, Mexico City 04510, DF, Mexico

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 07期

关键词：

Anaerobic membrane bioreactors; Biofouling; Extracellular polymeric substances; Temperature gradient gel electrophoresis (TGGE); 16S RIBOSOMAL-RNA; GRADIENT GEL-ELECTROPHORESIS; REVERSE-OSMOSIS MEMBRANE; BACTERIAL COMMUNITIES; BIOFILM FORMATION; DIVERSITY; OPERATION; ARCHAEA; GENES;

D O I：

10.1016/j.biortech.2011.01.007

中图分类号：

S2 [农业工程];

学科分类号：

082806 [农业信息与电气工程];

摘要：

The structure of the biofouling layers formed on a pilot-scale membrane-coupled upflow anaerobic sludge blanket bioreactor (UASB) used to treat urban wastewater was analyzed by scanning electron microscopy and electron-dispersive X-ray microanalysis. For comparison, control samples of the membranes were fed either UASB effluent or raw wastewater in a laboratory-scale experiment. Microbial diversity in the fouling materials was analyzed by temperature gradient gel electrophoresis (TGGE) combined with sequence analysis of partial 16S rRNA. Significant differences in structure of the Bacteria communities were observed amongst the different fouling layers analyzed in the UASB membranes, particularly following a chemical cleaning step (NaClO), while the Archaea communities retained more similarity in all samples. The main Bacteria populations identified were evolutively close to Firmicutes (42.3%) and Alphaproteobacteria (30.8%), while Archaea were mostly affiliated to the Methanosarcinales and Methanospirillaceae. Sphingomonadaceae-related bacteria and methanogenic Archaea were persistently found as components of biofouling, regardless of chemical cleaning. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：4618 / 4627

页数：10

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