Evaluation of fluorescent monochloro-s-triazine reagent for amino acid analysis

被引：4

作者：

Brückner, H ^{[1
]}

Wachsmann, M ^{[1
]}

机构：

[1] Univ Giessen, Interdisciplinary Res Ctr, Inst Nutr Sci, Dept Food Sci, D-35392 Giessen, Germany

来源：

CHROMATOGRAPHIA | 2003年 / 57卷 / Suppl 1期

关键词：

column liquid chromatography; amino acid analysis; pre-column derivatization; fluorescence labelling; cyanuric chloride;

D O I：

10.1007/BF02492096

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fluorescent reagent 2-chloro-4-methoxy-6-(4-methoxy-1-naphthyl)-[1,3,5]triazine (CMMNT) has been synthesized and used for derivatization of mixtures of a-amino acids. The derivatives were resolved on a Nucleosil(R) C-18 column using a linear gradient of 0.1% aqueous trifluoroacetic acid in acetonitrile and detected by their fluorescence at 415 nm using an excitation of 336 rim. Using a standard the linearity was determined from 0.13 - 26 pmol per amino acid derivative and the detection limit was 6.6 x 10(-2) pmol at a signal-to-noise ratio of 3.5. This approach was used for quantitative, amino acid analysis of total hydrolysates of synthetic peptides and for fluorescence labelling of casein.

引用

收藏

页码：S143 / S146

页数：4

相关论文

共 26 条

[1] IMMUNOFLUORESCENCE USING DICHLOROTRIAZINYLAMINOFLUORESCEIN (DTAF) .1. PREPARATION AND FRACTIONATION OF LABELED IGG [J].

BLAKESLEE, D ;

BAINES, MG .

JOURNAL OF IMMUNOLOGICAL METHODS, 1976, 13 (3-4) :305-320

[2] Liquid chromatographic separation of amino acid enantiomers on a silica-bonded chiral s-triazine column [J].

Bruckner, H ;

Wachsmann, M .

JOURNAL OF CHROMATOGRAPHY A, 1996, 728 (1-2) :447-454

[3] USE OF CHIRAL MONOHALO-S-TRIAZINE REAGENTS FOR THE LIQUID-CHROMATOGRAPHIC RESOLUTION OF DL-AMINO ACIDS [J].

BRUCKNER, H ;

STRECKER, B .

JOURNAL OF CHROMATOGRAPHY, 1992, 627 (1-2) :97-105

[4] USE OF CHROMOGENIC AND FLUORESCENT OXYCARBONYL CHLORIDES AS REAGENTS FOR AMINO-ACID-ANALYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BRUCKNER, H ;

LUPKE, M .

JOURNAL OF CHROMATOGRAPHY A, 1995, 697 (1-2) :295-307

[5] 1-ETHOXY-4-(DICHLORO-S-TRIAZINYL)NAPHTHALENE - FLUORESCENT REAGENT FOR CORTICOSTEROIDS [J].

CHAYEN, R ;

GOULD, S ;

HARELL, A ;

STEAD, CV .

ANALYTICAL BIOCHEMISTRY, 1971, 39 (02) :533-&

[6] SYNTHESIS OF A FLUORESCENT DERIVATIZING REAGENT, 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE, AND ITS APPLICATION FOR THE ANALYSIS OF HYDROLYSATE AMINO-ACIDS VIA HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

COHEN, SA ;

MICHAUD, DP .

ANALYTICAL BIOCHEMISTRY, 1993, 211 (02) :279-287

[7] 2-(9-ANTHRYL)ETHYL CHLOROFORMATE - A PRECOLUMN DERIVATIZING REAGENT FOR POLYAMINES DETERMINED BY LIQUID-CHROMATOGRAPHY AND FLUORESCENCE DETECTION [J].

FAULKNER, AJ ;

VEENING, H ;

BECKER, HD .

ANALYTICAL CHEMISTRY, 1991, 63 (03) :292-296

[8] 3-(dichloro-1,3,5-triazinyl)benz[f]isoindolo[1,2-b][1,3]thiazolidine as a derivatization reagent for bisphenols in high-performance liquid chromatography with argon laser-induced fluorescence detection [J].

Fujino, H ;

Yoshida, H ;

Nohta, H ;

Yamaguchi, M .

ANALYTICAL SCIENCES, 2000, 16 (09) :975-977

[9]

Godel H., 1992, LC GC INT, V5, P44

[10] FULLY AUTOMATED AMINO-ACID-ANALYSIS FOR PROTEIN AND PEPTIDE HYDROLYSATES BY PRECOLUMN DERIVATIZATION WITH 9-FLUORENYL METHYLCHLOROFORMATE AND 1-AMINOADAMANTANE [J].

GUSTAVSSON, B ;

BETNER, I .

JOURNAL OF CHROMATOGRAPHY, 1990, 507 :67-77