Molecular features of a defined genetic marker for the determination of the Porphyra tenera lineage

被引:25
作者
Kunimoto, M [1 ]
Kito, H
Mizukami, Y
Murase, N
Levine, I
机构
[1] Natl Fisheries Univ, Dept Food Sci, Yamaguchi 7596595, Japan
[2] Natl Fisheries Univ, Dept Appl Aquabiol, Yamaguchi 7596595, Japan
[3] Univ So Maine, LA Coll Nat & Appl Sci, Lewiston, ME 04240 USA
关键词
bangiaceae; identification; nucleotide sequence; Porphyra tenera; rhodophyta; ribosomal internal transcribed spacer 1; SSU rRNA gene;
D O I
10.1023/A:1025182508440
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Nucleotide sequences of the nuclear SSU rDNA and ITS1 are presented as a defined genetic marker for Porphyra tenera as a species. Exon nucleotide sequences were identical within all the P. tenera specimens. Intron nucleotide sequences varied between populations. The introns and ITS1 variations are presented as defined genetic markers to establish the Porphyra tenera strains. Wild-collected thalli identified by morphological systematics, from five populations of Porphyra tenera throughout Japan, were discriminated by comparing sequences of the various regions utilizing the results of this and previous studies.
引用
收藏
页码:337 / 343
页数:7
相关论文
共 9 条
[1]   A reassessment of the taxonomic status of Porphyra suborbiculata, Porphyra carolinensis and Porphyra lilliputiana (Bangiales, Rhodophyta) based on molecular and morphological data [J].
Broom, JE ;
Nelson, WA ;
Yarish, C ;
Jones, WA ;
Rosas, RA ;
Rosas, LEA .
EUROPEAN JOURNAL OF PHYCOLOGY, 2002, 37 (02) :227-235
[2]   Discrimination of Porphyra species based on small subunit ribosomal RNA gene sequence [J].
Kunimoto, M ;
Kito, H ;
Yamamoto, Y ;
Cheney, DP ;
Kaminishi, Y ;
Mizukami, Y .
JOURNAL OF APPLIED PHYCOLOGY, 1999, 11 (02) :203-209
[3]   Molecular divergence of the ssu rRNA gene and internal transcribed spacer 1 in Porphyra yezoensis (Rhodophyta) [J].
Kunimoto, M ;
Kito, H ;
Kaminishi, Y ;
Mizukami, Y ;
Murase, N .
JOURNAL OF APPLIED PHYCOLOGY, 1999, 11 (02) :211-216
[4]   Four new species of Porphyra (Bangiales, Rhodophyta) from the New Zealand region described using traditional characters and 18S rDNA sequence data [J].
Nelson, WA ;
Broom, JE ;
Farr, TJ .
CRYPTOGAMIE ALGOLOGIE, 2001, 22 (03) :263-284
[5]  
OLIVEIRA MC, 1994, MOL BIOL EVOL, V11, P195
[6]   THE NEIGHBOR-JOINING METHOD - A NEW METHOD FOR RECONSTRUCTING PHYLOGENETIC TREES [J].
SAITOU, N ;
NEI, M .
MOLECULAR BIOLOGY AND EVOLUTION, 1987, 4 (04) :406-425
[7]  
Tanaka T., 1952, MEM FAC FISH KAGOSHI, V2, P1
[8]  
TOKITA J, 1966, JPN J PHYCOL, V14, P146
[9]  
Ueda S., 1932, J. Imp. Fish. Inst., V28, P1