Healing of artificial fenestration defects by seeding of fibroblast-like cells derived from regenerated periodontal ligament in a dog:: A preliminary study

被引:46
作者
Dogan, A
Özdemir, A
Kubar, A
Oygür, T
机构
[1] Gazi Univ, Fac Dent, Dept Periodontol, TR-06510 Ankara, Turkey
[2] Gulhane Mil Med Acad, Dent Ctr, Ankara, Turkey
[3] Gulhane Mil Med Acad, Dept Virol, Ankara, Turkey
[4] Gazi Univ, Fac Dent, Dept Oral Pathol, TR-06510 Ankara, Turkey
来源
TISSUE ENGINEERING | 2003年 / 9卷 / 06期
关键词
D O I
10.1089/10763270360728099
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The purpose of this study was to assess the seeding of fibroblast-like cells to promote periodontal healing in artificial fenestration defects in a dog. Fibroblast-like cells were cultured by incubating regenerated periodontal ligament tissue, that had been surgically taken, underneath a Teflon membrane. Fenestration defects were surgically induced on the maxillary canine and first molar teeth at a spacing of 5 to 5 mm. Passage 4 cells (2 X 10(5) cells) in autologous blood coagulum were placed on root surfaces in two defects; the remaining two defects were used as controls. Healing was evaluated histomorphometrically on postoperative day 42. The main periodontal healing pattern consisted of connective tissue adaptation in three of the four specimens including one control, with cementum formation at 9-12%; one control specimen that exhibited 100% cementum formation. New bone formation was greater in the cell-seeding group (84%) compared with control (39%). In the cell-seeding group, one specimen exhibited total regeneration of bone (100%); however, the connective tissue located between newly formed bone and the root surface was observed to adapt to the dentin surface, with limited cementum formation. Seeding of cells from periodontal ligament may be promising to promote periodontal regeneration, but needs to be investigated in further studies.
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页码:1189 / 1196
页数:8
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