Development of species specific AFLP-derived SCAR marker for authentication of Panax japonicus C. A.!MEYER

被引：42

作者：

Choi, Yong-Eui ^{[1
]}

Ahn, Chang Ho ^{[1
]}

Kim, Bo-Bae ^{[2
]}

Yoon, Eui-Soo ^{[2
]}

机构：

[1] Kangwon Natl Univ, Div Forest Resources, Coll Forest Sci, Chunchon 200701, South Korea

[2] Kongju Natl Univ, Dept Biol, Kong Ju 314701, South Korea

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2008年 / 31卷 / 01期

关键词：

Panax japonica; molecular marker; sequence characterized amplified region marker;

D O I：

10.1248/bpb.31.135

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Panax japonicus is an important medicinal plant. The aim of this study was to develop species-specific molecular markers for P. japonicus. Amplified fragment length polymorphism (AFLP) was compared among P. japonicus, P. ginseng and R quinquefolius. A clear species-specific AFLP marker for P. japonicus was generated. After isolation and sequencing of the AFLP fragment, a DNA sequence (293 bp) was obtained and named JG 14. Oligonucleotide primer (23 mer) was designed for amplifying 191 bp of the sequence of JG14. PCR analysis folius) and P revealed a clear amplified band for P. japonicus but not in 3 other Panax species (P. ginseng, P. quinquefolius and P. ginseng). This sequence characterized amplified regions (SCAR) marker will be used for rapid authentication of Pjaponicits among other related Panax species. This is the first report of species-specific SCAR marker development in P. japonicus.

引用

页码：135 / 138

页数：4

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