Molecular cloning and functional expression of a protein-serine/threonine phosphatase from the hyperthermophilic archaeon Pyrodictium abyssi TAG11

被引：26

作者：

Mai, B

Frey, G

Swanson, RV

Mathur, EJ

Stetter, KO

机构：

[1] Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany

[2] Diversa Corp, San Diego, CA 92121 USA

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 16期

关键词：

D O I：

10.1128/JB.180.16.4030-4035.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An open reading frame coding for a putative protein-serine/threonine phosphatase was identified in the hyperthermophilic archaeon Pyrodictium abyssi TAG11 and named Py-PP1. Py-PP1 was expressed in Escherichia coli, purified from inclusion bodies, and biochemically characterized. The phosphatase gene is part of an operon which may pro, ide, for the first time, insight into a physiological role for archaeal protein phosphatases in vivo.

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页码：4030 / 4035

页数：6

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