Listerial invasion protein internalin B promotes entry into ileal Peyer's patches in vivo

被引：47

作者：

Chiba, Sayuri ^{[1
,2
]}

Nagai, Takeshi ^{[1
,2
]}

Hayashi, Toshiyuki ^{[1
]}

Baba, Yukiko ^{[1
,2
]}

Nagai, Shigenori ^{[1
,2
]}

Koyasu, Shigeo ^{[1
,3
]}

机构：

[1] Keio Univ, Dept Microbiol & Immunol, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan

[2] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020075, Japan

[3] Japan Soc Promot Sci, Res Ctr Sci Syst, Chiyoda Ku, Tokyo 1028472, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2011年 / 55卷 / 02期

关键词：

internalin B; Listeria monocytogenes; M cell; Peyer's patches; M-CELLS; MONOCYTOGENES; INFECTION; PATHOGEN; MODEL;

D O I：

10.1111/j.1348-0421.2010.00292.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Listeria monocytogenes (Lm) invades the host intestine using listerial invasion proteins, internalins. The in vivo role of internalin A (InlA) and internalin B (InlB) is reported here. Intragastric (i.g.) administration and ligated loop assays with delta inlB-Lm demonstrated that a lack of InlB significantly attenuates the invasive ability of Lm into various organs. On the other hand, InlAm-Lm expressing a mutant InlA with two substitutions, S192N and Y369S, which has been reported to increase the affinity of InlA to mouse E-cadherin, resulted in little increase in intestinal infection according to both ligated loop and i.g. infection assays. Lm preferentially enters ileal Peyer's patch (PP) via M cells and delta inlB-Lm showed severely reduced ability to invade though these cells. The present results reveal the importance of InlB, which accelerates listerial invasion into M cells on ileal PPs in vivo.

引用

页码：123 / 129

页数：7

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