In situ formation and scanning electrochemical microscopy assisted positioning of NO-sensors above human umbilical vein endothelial cells for the detection of nitric oxide release

被引：45

作者：

Pailleret, A ^{[1
]}

Oni, J ^{[1
]}

Reiter, S ^{[1
]}

Isik, S ^{[1
]}

Etienne, M ^{[1
]}

Bedioui, F ^{[1
]}

Schuhmann, W ^{[1
]}

机构：

[1] ENSCP, CNRS, UMR 7575, Lab Electrochim & Chim Analyt, F-75231 Paris 05, France

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2003年 / 5卷 / 10期

关键词：

nitric oxide; SECM; modified electrodes; HUVEC; NO sensor; LIVING CELLS; SENSITIVITY; OXIDATION;

D O I：

10.1016/j.elecom.2003.08.003

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Pt microelectrodes (50 mum diameter) were positioned by means of scanning electrochemical microscopy assisted z-approach curves and in situ modified with nickel tetrasulfonated phthalocyanine tetrasodium salt as electrocatalytic layer for the specific oxidative detection of nitric oxide. The thus modified electrodes were then moved over a layer of adherently growing human umbilical vein endothelial cells (HUVEC) in order to amperometrically detect nitric oxide (NO) released from the cells upon stimulation with bradykinin. This approach actually takes advantage of the use of SECM to define a sequential procedure that enables the in situ functionalisation of the SECM tip thus allowing to accurately control the separation between the functionalised SECM tip and the cell population. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：847 / 852

页数：6

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