Patch-clamp detection of neurotransmitters in capillary electrophoresis

被引:41
作者
Orwar, O
Jardemark, K
Jacobson, I
Moscho, A
Fishman, HA
Scheller, RH
Zare, RN
机构
[1] STANFORD UNIV,DEPT CHEM,STANFORD,CA 94305
[2] GOTHENBURG UNIV,DEPT ANAT & CELL BIOL,S-41390 GOTHENBURG,SWEDEN
[3] STANFORD UNIV,HOWARD HUGHES INST,DEPT MOL & CELLULAR PHYSIOL,STANFORD,CA 94305
关键词
D O I
10.1126/science.272.5269.1779
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Gamma-aminobutyrate acid, L-glutamate, and N-methyl-D-aspartate were separated by capillary electrophoresis and detected by the use of whole-cell and outside-out patch-clamp techniques on freshly dissociated rat olfactory interneurons. These neuroactive compounds could be identified from their electrophoretic migration times, unitary channel conductances, and power spectra that yielded corner frequencies and mean single-channel conductances characteristic for each of the different agonist-receptor interactions. This technique has the sensitivity to observe the opening of a single ion channel for agonists separated by capillary electrophoresis.
引用
收藏
页码:1779 / 1782
页数:4
相关论文
共 51 条
[1]   VOLTAGE CLAMP ANALYSIS OF ACETYLCHOLINE PRODUCED END-PLAT CURRENT FLUCTUATIONS AT FROG NEUROMUSCULAR-JUNCTION [J].
ANDERSON, CR ;
STEVENS, CF .
JOURNAL OF PHYSIOLOGY-LONDON, 1973, 235 (03) :655-691
[2]   EXTRACELLULAR ACIDIC SULFUR-CONTAINING AMINO-ACIDS AND GAMMA-GLUTAMYL PEPTIDES IN GLOBAL-ISCHEMIA - POSTISCHEMIC RECOVERY OF NEURONAL-ACTIVITY IS PARALLELED BY A TETRODOTOXIN-SENSITIVE INCREASE IN CYSTEINE SULFINATE IN THE CA1 OF THE RAT HIPPOCAMPUS [J].
ANDINE, P ;
ORWAR, O ;
JACOBSON, I ;
SANDBERG, M ;
HAGBERG, H .
JOURNAL OF NEUROCHEMISTRY, 1991, 57 (01) :230-236
[3]  
ASCHER P, 1988, J PHYSIOL-LONDON, V399, P207
[4]   NEURONAL BIOSENSORS [J].
BUCH, RM ;
RECHNITZ, GA .
ANALYTICAL CHEMISTRY, 1989, 61 (08) :A533-+
[5]  
BUFLER J, 1992, J COMP PHYSIOL A, V170, P153
[6]   GLUTAMATE NEUROTOXICITY AND DISEASES OF THE NERVOUS-SYSTEM [J].
CHOI, DW .
NEURON, 1988, 1 (08) :623-634
[7]   RELAXATION AND FLUCTUATIONS OF MEMBRANE CURRENTS THAT FLOW THROUGH DRUG-OPERATED CHANNELS [J].
COLQUHOUN, D ;
HAWKES, AG .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1977, 199 (1135) :231-262
[8]   RELEASE OF ENDOGENOUS EXCITATORY AMINO-ACIDS FROM TURTLE PHOTORECEPTORS [J].
COPENHAGEN, DR ;
JAHR, CE .
NATURE, 1989, 341 (6242) :536-539
[9]   NEUROTRANSMITTERS - NMDA RECEPTORS - DO GLIA HOLD THE KEY [J].
CULLCANDY, S .
CURRENT BIOLOGY, 1995, 5 (08) :841-843
[10]   MULTIPLE-CONDUCTANCE CHANNELS ACTIVATED BY EXCITATORY AMINO-ACIDS IN CEREBELLAR NEURONS [J].
CULLCANDY, SG ;
USOWICZ, MM .
NATURE, 1987, 325 (6104) :525-528