Prevention of neuronal cell damage induced by oxidative stress in-vitro:: effect of different Ginkgo biloba extracts

被引:53
作者
Guidetti, C
Paracchini, S
Lucchini, S
Cambieri, M
Marzatico, F
机构
[1] Univ Pavia, Dept Physiol & Pharmacol Sci, Pharmacol Sect, I-27100 Pavia, Italy
[2] Linnea SA, CH-6595 Riazzino, Switzerland
[3] Chimete SRL, Fine Chem Appl Res & Dev, I-15020 Pozzengo Monf, AL, Italy
关键词
D O I
10.1211/0022357011775442
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The effect of two different Ginkgo biloba extracts (GB1 and GB4) was studied in-vitro on cultured neurons exposed to oxidative stress caused by H2O2(50 mu mol L-1) and FeSO4(100 mu mol L-1). Only about 50% of the neurons were still viable at the end of the experiment (8 h) in control conditions, while the two extracts dose dependently increased the number of viable cells, in the concentration range 10-200 mug mL(-1). The two Ginkgo biloba extracts differed in their effect on hydroxyl-radical-scavenging capacity: GB1 and GB4 had an IC50 (50 % inhibiting concentration) value of 78 mug mL(-1) and 186 mug mL(-1), respectively. However, both extracts inhibited apoptosis in cortical neurons after oxidative stress in-vitro. These observations make one suppose that different preparations of Ginkgo biloba have quantitatively different actions and outline the importance of the contribution of apoptosis prevention toward their neuroprotective action.
引用
收藏
页码:387 / 392
页数:6
相关论文
共 15 条
[1]   Inhibition of serum deprivation- and staurosporine-induced neuronal apoptosis by Ginkgo biloba extract and some of its constituents [J].
Ahlemeyer, B ;
Möwes, A ;
Krieglstein, J .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1999, 367 (2-3) :423-430
[2]   Oxidative stress after acute and chronic application of beta-amyloid fragment 25-35 in cortical cultures [J].
Cafe, C ;
Torri, C ;
Bertorelli, L ;
Angeretti, N ;
Lucca, E ;
Forloni, G ;
Marzatico, F .
NEUROSCIENCE LETTERS, 1996, 203 (01) :61-65
[3]  
Chen C, 1999, BIOCHEM MOL BIOL INT, V47, P397
[4]   NEUROTOXICITY OF A PRION PROTEIN-FRAGMENT [J].
FORLONI, G ;
ANGERETTI, N ;
CHIESA, R ;
MONZANI, E ;
SALMONA, M ;
BUGIANI, O ;
TAGLIAVINI, F .
NATURE, 1993, 362 (6420) :543-546
[5]   OXIDATIVE STRESS-INDUCED APOPTOSIS PREVENTED BY TROLOX [J].
FORREST, VJ ;
KANG, YH ;
MCCLAIN, DE ;
ROBINSON, DH ;
RAMAKRISHNAN, N .
FREE RADICAL BIOLOGY AND MEDICINE, 1994, 16 (06) :675-684
[6]   THE DEOXYRIBOSE METHOD - A SIMPLE TEST-TUBE ASSAY FOR DETERMINATION OF RATE CONSTANTS FOR REACTIONS OF HYDROXYL RADICALS [J].
HALLIWELL, B ;
GUTTERIDGE, JMC ;
ARUOMA, OI .
ANALYTICAL BIOCHEMISTRY, 1987, 165 (01) :215-219
[7]   Proof of efficacy of the Ginkgo biloba special extract EGb 761 in outpatients suffering from mild to moderate primary degenerative dementia of the Alzheimer type or multi-infarct dementia (Reprinted from Pharmacopsychiat, vol 29, pg 47-56, 1996) [J].
Kanowski, S ;
Herrmann, WM ;
Stephan, K ;
Wierich, W ;
Horr, R .
PHYTOMEDICINE, 1997, 4 (01) :3-13
[8]   A placebo-controlled, double-blind, randomized trial of an extract of Ginkgo biloba for dementia [J].
LeBars, PL ;
Katz, MM ;
Berman, N ;
Itil, TM ;
Freedman, AM ;
Schatzberg, AF .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1997, 278 (16) :1327-1332
[9]   Clinical efficacy of Ginkgo biloba special extract EGb 761 in dementia of the Alzheimer type [J].
Maurer, K ;
Ihl, R ;
Dierks, T ;
Frolich, L .
JOURNAL OF PSYCHIATRIC RESEARCH, 1997, 31 (06) :645-655
[10]   Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals [J].
Ni, YC ;
Zhao, BL ;
Hou, JW ;
Xin, WJ .
NEUROSCIENCE LETTERS, 1996, 214 (2-3) :115-118