Epithelial Cells Lining Salivary Gland Ducts Are Early Target Cells of Severe Acute Respiratory Syndrome Coronavirus Infection in the Upper Respiratory Tracts of Rhesus Macaques

被引：261

作者：

Liu, Li ^{[3
]}

Wei, Qiang ^{[1
,2
]}

Alvarez, Xavier ^{[4
]}

Wang, Haibo ^{[3
]}

Du, Yanhua ^{[3
]}

Zhu, Hua ^{[1
,2
]}

Jiang, Hong ^{[1
,2
]}

Zhou, Jingying ^{[3
]}

Lam, Pokman ^{[3
]}

Zhang, Linqi ^{[5
]}

Lackner, Andrew ^{[4
]}

Qin, Chuan ^{[1
,2
]}

Chen, Zhiwei ^{[3
,6
,7
]}

机构：

[1] Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing 100021, Peoples R China

[2] Peking Union Med Coll, Beijing 100021, Peoples R China

[3] Univ Hong Kong, AIDS Inst, Li Ka Shing Fac Med, Hong Kong, Hong Kong, Peoples R China

[4] Tulane Natl Primate Res Ctr, Div Comparat Pathol, Covington, LA USA

[5] Tsinghua Univ, Comprehens AIDS Res Ctr, Beijing 100084, Peoples R China

[6] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[7] Univ Hong Kong, Res Ctr Infect & Immunol, Li Ka Shing Fac Med, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 08期

关键词：

ANGIOTENSIN-CONVERTING ENZYME; SIMIAN IMMUNODEFICIENCY VIRUS; SPIKE GLYCOPROTEIN DETERMINE; SARS-ASSOCIATED CORONAVIRUS; IN-SITU HYBRIDIZATION; FUNCTIONAL RECEPTOR; AFRICAN-GREEN; ANIMAL-MODEL; PATHOGENESIS; LUNG;

D O I：

10.1128/JVI.02292-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. The source of high viral loads in saliva, however, remains elusive. Here we investigate the early target cells of infection in the entire array of respiratory tissues in Chinese macaques after intranasal inoculations with a single-cycle pseudotyped virus and a pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2(+)) cells were widely distributed in the upper respiratory tract, and ACE2(+) epithelial cells lining salivary gland ducts were the early target cells productively infected. Our findings also have implications for SARS-CoV early diagnosis and prevention.

引用

页码：4025 / 4030

页数：6

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