ZmPUMP encodes a maize mitochondrial uncoupling protein that is induced by oxidative stress

被引：18

作者：

Brandalise, M

Maia, ID

Borecky, J

Vercesi, AE

Arruda, P

机构：

[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil

[2] Univ Estadual Campinas, Dept Patol Clin, NMCE, FCM, BR-13083970 Campinas, SP, Brazil

来源：

PLANT SCIENCE | 2003年 / 165卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

plant uncoupling mitochondrial protein; mitochondria; oxidative stress; maize;

D O I：

10.1016/S0168-9452(03)00159-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone (designated ZmPUMP) encoding an uncoupling protein (UCP) from maize (Zea mays) was identified by searching for homologous sequences among the expressed sequence tags of the GenBank database. The ZmPUMP cDNA contains a single open reading frame of 933 nucleotides encoding 3 10 amino acids. Several features identified the predicted ZmPUMP protein as a member of the mitochondrial UCP subfamily of mitochondrial carriers. Expression studies demonstrated that ZmPUMP is ubiquitously expressed in maize tissues and its transcript level is not altered in early stages of embryo germination. In contrast to known UCP genes, ZmPUMP is not responsive to cold stress. Instead its expression is increased in response to H2O2- or menadione-induced oxidative stress. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：329 / 335

页数：7

共 27 条

[1] Cyanide-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respiration during postharvest ripening of tomato fruit [J].