Detection of banned meat and bone meal in feedstuffs by near-infrared microscopic analysis of the dense sediment fraction

In this paper we present an alternative method for detection of meat and bone meal (MBM) in feed-stuffs by near-infrared microscopic (NIRM) analysis of the particles in the sediment fraction (dense fraction (d > 1.62) from dichloroethylene) of compound feeds. To apply this method the particles of the sediment fraction are spread on a sample holder and presented to the NIR microscope. By using the pointer of the microscope the infrared beam is focussed on each particle and the NIR spectrum (1112-2500 nm) is collected. This method can be used to detect the presence of MBM at concentrations as low as 0.05% mass fraction. When results from the NIRM method were compared with the classical microscopic method, a coefficient of determination (R-2) of 0.87 was obtained. The results of this study demonstrated that this method could be proposed as a complementary tool for the detection of banned MBM in feedstuffs by reinforcement of the monitoring of feeds.