Bioelectrochemistry of glucose oxidase immobilized on ferrocene encapsulated ormosil modified electrode

A novel enzyme electrode based on Pd-linked ormosil material having encapsulated ferrocene is reported. The ferrocene encapsulated ormosil is developed using Pd-glycidoxypropyltrimethoxysilane, ferrocene monocarboxylic, trimethoxysilnane, and HCl. The resulting ormosil is characterized by scanning electron micrograph (SEM) and cyclic voltammetric (CV) measurements. A linear relation between peak current and square root of scan rate where the linear line passes through origin suggests reversible electrochemistry of ormosil encapsulated ferrocene which is one of the novel finding of the present investigation. The glucose oxidase is immobilized over the ferrocene encapsulated ormosil modified electrode using polyvinyl alcohol. The electrocatalytic response of immobilized glucose oxidase over ferrocene encapsulated Pd-linked ormosil is observed and the results are reported which suggest that the new ormosil matrix behaves as solid solution. The performance, stability, and reproducibility of new enzyme electrode and the contribution of palladium linkage together with encapsulated ferrocene are reported. (C) 2001 Elsevier Science B.V. All rights reserved.