Restricted transcription from sigma H or phosphorylated Spo0A dependent promoters in the temperature-sensitive secA341 mutant of Bacillus subtilis

被引:8
作者
Asai, K
Fujita, M
Kawamura, F
Takahashi, H
Kobayashi, Y
Sadaie, Y [1 ]
机构
[1] Natl Inst Genet, Radioisotope Ctr, Mishima, Shizuoka 4118540, Japan
[2] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan
[3] Tokyo Univ Agr & Technol, Lab Mol Biol & Microbial Chem, Fuchu, Tokyo 1830054, Japan
关键词
Bacillus subtilis; sigma(H) promoter; sporulation;
D O I
10.1271/bbb.62.1707
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The temperature-sensitive secA341 mutation of Bacillus subtilis affects sporulation and sporulation-associated events as well as protein secretion and cell septation. With lacZ or bgaB fusion genes, we examined the expression of the early sporulation genes in the mutant strain. Transcriptional expression of sigma(H) dependent kinA, spo0A (Ps), phrC, spoVG, and citG (p2) genes was blocked by the secA341 mutation at 37 degrees C. On the other hand, neither repression of the abrB gene nor induction of the spo0H (sigma(H)) gene was affected. Active RNA polymerase containing sigma(H) was, however, found to be produced in the mutant cells. Expression of the phosphorylated Spo0A dependent spoIIG operon was also blocked. Thus the secA341 mutation blocks some step(s) or factor(s) required for sigma(H)-dependent transcription in vivo.
引用
收藏
页码:1707 / 1713
页数:7
相关论文
共 51 条
[1]   REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].
ANAGNOSTOPOULOS, C ;
SPIZIZEN, J .
JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&
[2]   SECA IS REQUIRED FOR 3 DISTINCT STAGES OF SPORULATION IN BACILLUS-SUBTILIS [J].
ASAI, K ;
KAWAMURA, F ;
HIRATA, A ;
YOSHIKAWA, H ;
TAKAHASHI, H .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1993, 39 (06) :583-596
[3]   Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene [J].
Asai, K ;
Kawamura, F ;
Sadaie, Y ;
Takahashi, H .
JOURNAL OF BACTERIOLOGY, 1997, 179 (02) :544-547
[4]   EXPRESSION OF KINA AND ACCUMULATION OF SIGMA(H) AT THE ONSET OF SPORULATION IN BACILLUS-SUBTILIS [J].
ASAI, K ;
KAWAMURA, F ;
YOSHIKAWA, H ;
TAKAHASHI, H .
JOURNAL OF BACTERIOLOGY, 1995, 177 (22) :6679-6683
[5]   PHOSPHORYLATION OF BACILLUS-SUBTILIS TRANSCRIPTION FACTOR SPOOA STIMULATES TRANSCRIPTION FROM THE SPOIIG PROMOTER BY ENHANCING BINDING TO WEAK OA BOXES [J].
BALDUS, JM ;
GREEN, BD ;
YOUNGMAN, P ;
MORAN, CP .
JOURNAL OF BACTERIOLOGY, 1994, 176 (02) :296-306
[6]   PHOSPHORYLATION OF SPOOA ACTIVATES ITS STIMULATION OF IN-VITRO TRANSCRIPTION FROM THE BACILLUS-SUBTILIS-SPOLLG OPERON [J].
BIRD, TH ;
GRIMSLEY, JK ;
HOCH, JA ;
SPIEGELMAN, GB .
MOLECULAR MICROBIOLOGY, 1993, 9 (04) :741-749
[7]   INITIATION OF SPORULATION IN BACILLUS-SUBTILIS IS CONTROLLED BY A MULTICOMPONENT PHOSPHORELAY [J].
BURBULYS, D ;
TRACH, KA ;
HOCH, JA .
CELL, 1991, 64 (03) :545-552
[8]   NEW RNA-POLYMERASE SIGMA-FACTOR UNDER SPOO CONTROL IN BACILLUS-SUBTILIS [J].
CARTER, HL ;
MORAN, CP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9438-9442
[9]  
CARTER HL, 1990, GENE, V96, P101
[10]  
CASADABAN MJ, 1983, METHOD ENZYMOL, V100, P293