Decolorization of azo dye in a FBR reactor using immobilized bacteria

被引:22
作者
Wu, JY [1 ]
Hwang, SCJ
Chen, CT
Chen, KC
机构
[1] Da Yeh Univ, Dept Bioind Technol, Changhua 515, Taiwan
[2] Chung Hua Univ, Dept Civil Engn, Hsinchu 300, Taiwan
[3] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan
关键词
azo dye; decolorization; PVA; immobilized-cell beads; fluidized-bed reactor;
D O I
10.1016/j.enzmictec.2005.02.012
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Decolorization of azo dye (RED RBN) was carried out experimentally in a liquid-solid fluidized bed reactor (FBR) using polyvinyl alcohol (PVA)-immobilized cell beads as support carriers. The effects of various operating conditions such as bed expansion, cell bead number density, initial dye concentration, hydraulic retention time (HRT), and diameters of immobilized cell beads on the decolorization of azo dye were demonstrated experimentally. It was found that azo dye degradation time reaching initially a steady state decreased with an increase in bed expansion, cell bead number density as well as HRT. The mean cell residence time (theta(C)) in FBR using PVA-immobilized cell beads increased insignificantly from 1014.1 to 1014.9 days as the HRT increased from 3 to 24 h, and thus the impact of convectional reduced theta(C) could be minimized by using the same polymer as support carriers. In addition, a mathematical model was used to describe the simultaneous diffusion and reaction of azo dye in the FBR. The internal mass-transfer resistance, rather than the film diffusion resistance, played an important role in azo dye utilization in FBR when film modulus (m(f)) was smaller than 1. The model was employed to analyze and predict both the k(L) value and the operational efficiency of FBR (which was running in a well-mixed state) in azo dye biodegradation using immobilized cell beads during a steady-state operation. The simulated results corresponded satisfactorily with the experimental data in the azo dye concentration ranging up to 2200 mg L-1. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:102 / 112
页数:11
相关论文
共 28 条
[1]   DESIGN OF FLUIDIZED-BED FERMENTERS [J].
ANDREWS, GF ;
PRZEZDZIECKI, J .
BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (06) :802-810
[2]   Microbial decolorization of textile-dye-containing effluents: A review [J].
Banat, IM ;
Nigam, P ;
Singh, D ;
Marchant, R .
BIORESOURCE TECHNOLOGY, 1996, 58 (03) :217-227
[3]   MODELING GLUCOSE FRUCTOSE ISOMERIZATION WITH IMMOBILIZED GLUCOSE-ISOMERASE IN FIXED AND FLUID BED REACTORS [J].
BECK, M ;
KIESSER, T ;
PERRIER, M ;
BAUER, W .
CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 1986, 64 (04) :553-560
[4]  
CARCIACALDERON D, 1998, BIOTECHNOL BIOENG, V57, P136
[5]  
CARLIELL CM, 1995, WATER SA, V21, P61
[6]   IMMOBILIZATION OF MICROORGANISMS WITH PHOSPHORYLATED POLYVINYL-ALCOHOL (PVA) GEL [J].
CHEN, KC ;
LIN, YF .
ENZYME AND MICROBIAL TECHNOLOGY, 1994, 16 (01) :79-83
[7]   Decolorization of azo dye using PVA-immobilized microorganisms [J].
Chen, KC ;
Wu, JY ;
Huang, CC ;
Liang, YM ;
Hwang, SCJ .
JOURNAL OF BIOTECHNOLOGY, 2003, 101 (03) :241-252
[8]   Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA-immobilized cell beads [J].
Chen, KC ;
Wu, JY ;
Yang, WB ;
Hwang, SCJ .
BIOTECHNOLOGY AND BIOENGINEERING, 2003, 83 (07) :821-832
[9]   Decolorization of the textile dyes by newly isolated bacterial strains [J].
Chen, KC ;
Wu, JY ;
Liou, DJ ;
Hwang, SCJ .
JOURNAL OF BIOTECHNOLOGY, 2003, 101 (01) :57-68
[10]   Simultaneous carbon-nitrogen removal in wastewater using phosphorylated PVA-immobilized microorganisms [J].
Chen, KC ;
Lee, SC ;
Chin, SC ;
Houng, JY .
ENZYME AND MICROBIAL TECHNOLOGY, 1998, 23 (05) :311-320