Detection of active butyrate-degrading microorganisms in methanogenic sludges by RNA-based stable isotope probing

被引：27

作者：

Hatamoto, Masashi ^{[2
]}

Imachi, Hiroyuki ^{[1
,2
]}

Yashiro, Yuto ^{[2
]}

Ohashi, Akiyoshi ^{[2
,3
]}

Harada, Hideki ^{[2
,4
]}

机构：

[1] Japan Agcy Marine Earth Sci & Technol, Subground Animalcule Retrieval Program, Extremobiosphere Res Ctr, Yokosuka, Kanagawa 2370061, Japan

[2] Nagaoka Univ Technol, Dept Environm Syst Engn, Niigata 9402188, Japan

[3] Hiroshima Univ, Dept Social & Environm Engn, Higashihiroshima 7398527, Japan

[4] Tohoku Univ, Dept Civil Engn, Sendai, Miyagi 9808579, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 11期

关键词：

D O I：

10.1128/AEM.00045-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Butyrate-degrading bacteria in four methanogenic sludges were studied by RNA-based stable isotope probing. Bacterial populations in the C-13-labeled rRNA fractions were distinct from unlabeled fractions, and Syntrophaceae species, Tepidanaerobacter sp., and Clostridium spp. dominated. These results suggest that diverse microbes were active in butyrate degradation under methanogenic conditions.

引用

页码：3610 / 3614

页数：5

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