Reverse transcriptase-polymerase chain reaction and immunocytochemistry from bone marrow and peripheral blood for detection of microdisseminated prostate cancer. A comparative analysis

被引：3

作者：

Borchers, H ^{[1
]}

Wolff, JM ^{[1
]}

Brehmer, B ^{[1
]}

Beniers, AJMC ^{[1
]}

Jakse, G ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Univ Klinikum, Urol Klin, D-52057 Aachen, Germany

来源：

UROLOGE A | 2000年 / 39卷 / 06期

关键词：

prostatic carcinoma; reverse transcriptase-polymerase chain reaction immunocytochemistry; prostate-specific antigen; cytokeratin-18;

D O I：

10.1007/s001200050412

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Reverse transcriptase-polymerase chain reaction (RT-PCR) assay for prostate-specific antigen and immunocytochemistry for cytokeratin-18 (CK-18) are tests for the detection of microdisseminated carcinoma of the prostate. Bone marrow aspirates and peripheral venous blood from 50 patients with clinically organ-confined prostate cancer were examined. The rate of positive results was independent of the pT stage, serum PSA, and previous antiandrogen treatment. RT-PCR and immunocytochemistry have to be tested under standardized conditions in prospective trials,and the results have to be compared to the serum PSA follow-up.

引用

页码：565 / 571

页数：9

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