Yokonolide B, a novel inhibitor of auxin action, blocks degradation of AUX/IAA factors

被引:49
作者
Hayashi, K
Jones, AM
Ogino, K
Yamazoe, A
Oono, Y
Inoguchi, M
Kondo, H
Nozaki, H
机构
[1] Okayama Univ Sci, Dept Biochem, Okayama 7000005, Japan
[2] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[3] Japan Atom Energy Res Inst, Dept Ion Beam Appl Biol, Takasaki, Gumma 3701292, Japan
关键词
D O I
10.1074/jbc.M300299200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yokonolide B (YkB; also known as A82548A), a spiroketal-macrolide, was isolated from Streptomyces diastatochromogenes B59 in a screen for inhibitors of beta-glucoronidase expression under the control of an auxin-responsive promoter in Arabidopsis. YkB inhibits the expression of auxin-inducible genes as shown using native and synthetic auxin promoters as well as using expression profiling of 8,300 Arabidopsis gene probes but does not affect expression of an abscisic acid- and a gibberellin A(3)-inducible gene. The mechanism of action of YkB is to block AUX/IAA protein degradation; however, YkB is not a general proteasome inhibitor. YkB blocks auxin-dependent cell division and auxin-regulated epinastic growth mediated by auxin-binding protein 1. Gain of function mutants such as shy2-2, slr1, and axr2-1 encoding AUX/IAA transcriptional repressors and loss of function mutants encoding components of the ubiquitin-proteolytic pathway such as axr1-3 and tir1-1, which display increased AUX/IAAs protein stability, are less sensitive to YkB, although axr1 and tir1 mutants were sensitive to MG132, a general proteasome inhibitor, consistent with a site of action downstream of AXR1 and TIR. YkB-treated seedlings displayed similar phenotypes as dominant AUX/IAA mutants. Taken together, these results indicate that YkB acts to block AUX/IAA protein degradation upstream of AXR and TIR, links a shared element upstream of AUX/IAA protein stability to auxin-induced cell division/elongation and to auxin-binding protein 1, and provides a new tool to dissect auxin signal transduction.
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收藏
页码:23797 / 23806
页数:10
相关论文
共 57 条
[1]   THE PS-IAA4/5-LIKE FAMILY OF EARLY AUXIN-INDUCIBLE MESSENGER-RNAS IN ARABIDOPSIS-THALIANA [J].
ABEL, S ;
NGUYEN, MD ;
THEOLOGIS, A .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 251 (04) :533-549
[2]   Early genes and auxin action [J].
Abel, S ;
Theologis, A .
PLANT PHYSIOLOGY, 1996, 111 (01) :9-17
[3]   EARLY AUXIN-INDUCED GENES ENCODE SHORT-LIVED NUCLEAR PROTEINS [J].
ABEL, S ;
OELLER, PW ;
THEOLOGIS, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) :326-330
[4]   INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE [J].
BACHMAIR, A ;
FINLEY, D ;
VARSHAVSKY, A .
SCIENCE, 1986, 234 (4773) :179-186
[5]   IDENTIFICATION OF THE AUXIN-RESPONSIVE ELEMENT, AUXRE, IN THE PRIMARY INDOLEACETIC ACID-INDUCIBLE GENE, PS-IAA4/5, OF PEA (PISUM-SATIVUM) [J].
BALLAS, N ;
WONG, LM ;
THEOLOGIS, A .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 233 (04) :580-596
[6]   Auxin transport promotes Arabidopsis lateral root initiation [J].
Casimiro, I ;
Marchant, A ;
Bhalerao, RP ;
Beeckman, T ;
Dhooge, S ;
Swarup, R ;
Graham, N ;
Inzé, D ;
Sandberg, G ;
Casero, PJ ;
Bennett, M .
PLANT CELL, 2001, 13 (04) :843-852
[7]   The role of auxin-binding protein 1 in the expansion of tobacco leaf cells [J].
Chen, JG ;
Shimomura, S ;
Sitbon, F ;
Sandberg, G ;
Jones, AM .
PLANT JOURNAL, 2001, 28 (06) :607-617
[8]   Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis [J].
Cheong, YH ;
Chang, HS ;
Gupta, R ;
Wang, X ;
Zhu, T ;
Luan, S .
PLANT PHYSIOLOGY, 2002, 129 (02) :661-677
[9]   Characterization of the response of the arabidopsis response regulator gene family to cytokinin [J].
D'Agostino, IB ;
Deruère, J ;
Kieber, JJ .
PLANT PHYSIOLOGY, 2000, 124 (04) :1706-1717
[10]   The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1 [J].
del Pozo, JC ;
Estelle, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15342-15347