Cytological effects of platelet-derived growth factor on mitochondrial ultrastructure in fibroblasts

被引:19
作者
Gosslau, A [1 ]
Dittrich, W [1 ]
Willig, A [1 ]
Jaros, PP [1 ]
机构
[1] Univ Oldenburg, Abt Zoophysiol, Fachbereich 7, D-29111 Oldenburg, Germany
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY | 2001年 / 128卷 / 02期
关键词
mitochondria; platelet-derived growth factor; cell cycle; G1-phase; NRK-49; fibroblasts; wound healing; electron microscopy; stereological analysis;
D O I
10.1016/S1095-6433(00)00303-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The goal of this study was to evaluate morphofunctional changes in mitochondrial ultrastructure after platelet-derived growth factor application in fibroblasts as an indicator of mitochondrial activation in processes like wound healing. NRK-49F fibroblasts were synchronized, incubated with PDGF (platelet-derived growth factor) and studied by electron microscopy. Volume density (V-c), numerical density (N-v) and surface density (S-v) were measured by stereological analysis, Application of PDGF on NRK-49F caused an increase in mitochondrial volume density by 57% and surface area of cristae per mitochondrion by 65%. The numerical density of the mitochondria was decreased in the PDGF-treated cells by 23%, but at the same time their mean volume was increased. Furthermore, the mitochondria had a complex and highly variable shape both in control and PDGF-treated cells, possibly indicating the existence of a mitochondrial reticulum. The results demonstrated that biochemically active membrane systems in fibroblast mitochondria are enlarged as a direct effect of small doses of platelet-derived growth factor and support the concept that this factor and related peptides serve as mitogens for connective tissue forming cells, Thus, in mitogenic processes like wound healing, the high energy demand of fibroblasts is provided by the increase of the inner surface of mitochondria, (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:241 / 249
页数:9
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