共 41 条
Initial steps of signal generation in photoactive yellow protein revealed with femtosecond mid-infrared spectroscopy
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van Wilderen, LJGW
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机构: Vrije Univ Amsterdam, Fac Sci, NL-1081 HV Amsterdam, Netherlands

Larsen, DS
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机构: Vrije Univ Amsterdam, Fac Sci, NL-1081 HV Amsterdam, Netherlands

van der Horst, MA
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机构: Vrije Univ Amsterdam, Fac Sci, NL-1081 HV Amsterdam, Netherlands

van Stokkum, IHM
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机构: Vrije Univ Amsterdam, Fac Sci, NL-1081 HV Amsterdam, Netherlands

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[1] Vrije Univ Amsterdam, Fac Sci, NL-1081 HV Amsterdam, Netherlands
[2] Univ Amsterdam, Microbiol Lab, Swammerdam Inst Life Sci, NL-1010 WV Amsterdam, Netherlands
关键词:
D O I:
10.1021/bi034878p
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Photoactive yellow protein (PYP) is a bacterial blue light sensor that induces Halorhodospira halophila to swim away from intense blue light. Light absorption by PYP's intrinsic chromophore, p-coumaric acid, leads to the initiation of a photocycle that comprises several distinct intermediates. Here we describe the initial structural changes of the chromophore and its nearby amino acids, using visible pump/mid-infrared probe spectroscopy. Upon photoexcitation, the trans bands of the chromophore are bleached, and shifts of the phenol ring bands occur. The latter are ascribed to charge translocation, which probably plays an essential role in driving the trans to cis isomerization process. We conclude that breaking of the hydrogen bond of the chromophore's C=O group with amino acid Cys69 and formation of a stable cis ground state occur in similar to2 ps. Dynamic changes also include rearrangements of the hydrogen-bonding network of the amino acids around the chromophore. Relaxation of the coumaryl tail of the chromophore occurs in 0.9-1 ns, which event we identify with the I-0 to I-1 transition observed in visible spectroscopy.
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页码:10054 / 10059
页数:6
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共 41 条
[1]
COMPLETE CHEMICAL-STRUCTURE OF PHOTOACTIVE YELLOW PROTEIN - NOVEL THIOESTER-LINKED 4-HYDROXYCINNAMYL CHROMOPHORE AND PHOTOCYCLE CHEMIST
[J].
BACA, M
;
BORGSTAHL, GEO
;
BOISSINOT, M
;
BURKE, PM
;
WILLIAMS, DR
;
SLATER, KA
;
GETZOFF, ED
.
BIOCHEMISTRY,
1994, 33 (48)
:14369-14377

BACA, M
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

BORGSTAHL, GEO
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

BOISSINOT, M
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

BURKE, PM
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

WILLIAMS, DR
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

SLATER, KA
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

GETZOFF, ED
论文数: 0 引用数: 0
h-index: 0
机构:
SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA
[2]
The primary events in the photoactivation of yellow protein
[J].
Baltuska, A
;
vanStokkum, IHM
;
Kroon, A
;
Monshouwer, R
;
Hellingwerf, KJ
;
vanGrondelle, R
.
CHEMICAL PHYSICS LETTERS,
1997, 270 (3-4)
:263-266

Baltuska, A
论文数: 0 引用数: 0
h-index: 0
机构: VRIJE UNIV AMSTERDAM,FAC PHYS & ASTRON,AMSTERDAM,NETHERLANDS

vanStokkum, IHM
论文数: 0 引用数: 0
h-index: 0
机构: VRIJE UNIV AMSTERDAM,FAC PHYS & ASTRON,AMSTERDAM,NETHERLANDS

Kroon, A
论文数: 0 引用数: 0
h-index: 0
机构: VRIJE UNIV AMSTERDAM,FAC PHYS & ASTRON,AMSTERDAM,NETHERLANDS

Monshouwer, R
论文数: 0 引用数: 0
h-index: 0
机构: VRIJE UNIV AMSTERDAM,FAC PHYS & ASTRON,AMSTERDAM,NETHERLANDS

Hellingwerf, KJ
论文数: 0 引用数: 0
h-index: 0
机构: VRIJE UNIV AMSTERDAM,FAC PHYS & ASTRON,AMSTERDAM,NETHERLANDS

vanGrondelle, R
论文数: 0 引用数: 0
h-index: 0
机构: VRIJE UNIV AMSTERDAM,FAC PHYS & ASTRON,AMSTERDAM,NETHERLANDS
[3]
Structure of the I1 early intermediate of photoactive yellow protein by FTIR spectroscopy
[J].
Brudler, R
;
Rammelsberg, R
;
Woo, TT
;
Getzoff, ED
;
Gerwert, K
.
NATURE STRUCTURAL BIOLOGY,
2001, 8 (03)
:265-270

Brudler, R
论文数: 0 引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Rammelsberg, R
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Woo, TT
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Getzoff, ED
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Gerwert, K
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[4]
Coupling of hydrogen bonding to chromophore conformation and function in photoactive yellow protein
[J].
Brudler, R
;
Meyer, TE
;
Genick, UK
;
Devanathan, S
;
Woo, TT
;
Millar, DP
;
Gerwert, K
;
Cusanovich, MA
;
Tollin, G
;
Getzoff, ED
.
BIOCHEMISTRY,
2000, 39 (44)
:13478-13486

Brudler, R
论文数: 0 引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Meyer, TE
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Genick, UK
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Devanathan, S
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Woo, TT
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Millar, DP
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Gerwert, K
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Cusanovich, MA
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Tollin, G
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Getzoff, ED
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[5]
Femtosecond spectroscopic observations of initial intermediates in the photocycle of the photoactive yellow protein from Ectothiorhodospira halophila
[J].
Devanathan, S
;
Pacheco, A
;
Ujj, L
;
Cusanovich, M
;
Tollin, G
;
Lin, S
;
Woodbury, N
.
BIOPHYSICAL JOURNAL,
1999, 77 (02)
:1017-1023

Devanathan, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA

Pacheco, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA

Ujj, L
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA

Cusanovich, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA

Tollin, G
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA

Lin, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA

Woodbury, N
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Dept Biochem, Tucson, AZ 85721 USA
[6]
Structure at 0.85 Å resolution of an early protein photocycle intermediate
[J].
Genick, UK
;
Soltis, SM
;
Kuhn, P
;
Canestrelli, IL
;
Getzoff, ED
.
NATURE,
1998, 392 (6672)
:206-209

Genick, UK
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Soltis, SM
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Kuhn, P
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Canestrelli, IL
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

Getzoff, ED
论文数: 0 引用数: 0
h-index: 0
机构: Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[7]
Structure of a protein photocycle intermediate by millisecond time-resolved crystallography
[J].
Genick, UK
;
Borgstahl, GEO
;
Ng, K
;
Ren, Z
;
Pradervand, C
;
Burke, PM
;
Srajer, V
;
Teng, TY
;
Schildkamp, W
;
McRee, DE
;
Moffat, K
;
Getzoff, ED
.
SCIENCE,
1997, 275 (5305)
:1471-1475

Genick, UK
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Borgstahl, GEO
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Ng, K
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Ren, Z
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Pradervand, C
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Burke, PM
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Srajer, V
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Teng, TY
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Schildkamp, W
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

McRee, DE
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Moffat, K
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA

Getzoff, ED
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA UNIV CHICAGO, DEPT BIOCHEM & MOL BIOL, CHICAGO, IL 60637 USA
[8]
Photoequilibrium in the primary steps of the photoreceptors phytochrome A and photoactive yellow protein
[J].
Gensch, T
;
Hellingwerf, KJ
;
Braslavsky, SE
;
Schaffner, K
.
JOURNAL OF PHYSICAL CHEMISTRY A,
1998, 102 (28)
:5398-5405

Gensch, T
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Strahlenchem, D-45413 Mulheim, Germany

Hellingwerf, KJ
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Strahlenchem, D-45413 Mulheim, Germany

Braslavsky, SE
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Strahlenchem, D-45413 Mulheim, Germany

Schaffner, K
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Strahlenchem, D-45413 Mulheim, Germany
[9]
The primary photoreaction of photoactive yellow protein (PYP): anisotropy changes and excitation wavelength dependence
[J].
Gensch, T
;
Gradinaru, CC
;
van Stokkum, IHM
;
Hendriks, J
;
Hellingwerf, KJ
;
van Grondelle, R
.
CHEMICAL PHYSICS LETTERS,
2002, 356 (3-4)
:347-354

论文数: 引用数:
h-index:
机构:

Gradinaru, CC
论文数: 0 引用数: 0
h-index: 0
机构: Free Univ Amsterdam, Dept Phys & Astron, Fac Sci, Inst Mol Biol Sci, NL-1081 HV Amsterdam, Netherlands

van Stokkum, IHM
论文数: 0 引用数: 0
h-index: 0
机构: Free Univ Amsterdam, Dept Phys & Astron, Fac Sci, Inst Mol Biol Sci, NL-1081 HV Amsterdam, Netherlands

Hendriks, J
论文数: 0 引用数: 0
h-index: 0
机构: Free Univ Amsterdam, Dept Phys & Astron, Fac Sci, Inst Mol Biol Sci, NL-1081 HV Amsterdam, Netherlands

Hellingwerf, KJ
论文数: 0 引用数: 0
h-index: 0
机构: Free Univ Amsterdam, Dept Phys & Astron, Fac Sci, Inst Mol Biol Sci, NL-1081 HV Amsterdam, Netherlands

论文数: 引用数:
h-index:
机构:
[10]
Signal transduction in the photoactive yellow protein. I. Photon absorption and the isomerization of the chromophore
[J].
Groenhof, G
;
Lensink, MF
;
Berendsen, HJC
;
Snijders, JG
;
Mark, AE
.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,
2002, 48 (02)
:202-211

Groenhof, G
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands

Lensink, MF
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands

Berendsen, HJC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands

Snijders, JG
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands

Mark, AE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biophys Chem, NL-9747 AG Groningen, Netherlands