Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates

被引:47
作者
Dreher, F
Modjtahedi, BS
Modjtahedi, SP
Maibach, HI
机构
[1] Univ Calif San Francisco, Dept Dermatol, San Francisco, CA 94143 USA
[2] TOPALIX, Top Prod Dev, San Francisco, CA USA
[3] Univ Calif Davis, Davis, CA 95616 USA
[4] Univ Calif Los Angeles, Sch Med, Los Angeles, CA USA
关键词
lowry assay; homogeneity of removal; microassay; drug discovery;
D O I
10.1111/j.1600-0846.2005.00103.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background/aims: Examination of stratum corneum (SC) content with tape stripping and a colorimetric method is increasingly used. We examined the possible use of microplates in tandem with a colorimetric method to examine SC removed with tape stripping. As a corollary to this examination, the homogeneity of tape strips was examined. Method: The commonly used Lowry assay was adapted for 96-well plates. Tapes were divided into four regions and sample disks of 5 mm diameter were taken from each and analyzed for SC mass using the adapted Lowry assay. Results: Homogeneity of SC removal over different areas across a tape strip is limited. Conclusion: Quantification of SC by means of a 96-well microplate-based colorimetric method is feasible and shortens the time of analysis. However, when using D-Squame(R) tape disks, SC removal on a limited area of the tape is not predictive for SC removal on the entire tape as removal is inhomogenous. Therefore, SC protein extraction should be performed on a large enough area, eventually on the entire tape when quantifying SC mass removed by tape stripping.
引用
收藏
页码:97 / 101
页数:5
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