Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates

被引：85

作者：

Gimeno, Mariona ^{[1
,2
]}

Darwich, Laila ^{[1
,2
]}

Diaz, Ivan ^{[2
]}

de la Torre, Eugenia ^{[2
]}

Pujols, Joan ^{[2
]}

Martin, Marga ^{[1
,2
]}

Inumaru, Shigeki ^{[3
]}

Cano, Esmeralda ^{[2
]}

Domingo, Mariano ^{[1
,2
]}

Montoya, Maria ^{[2
]}

Mateu, Enric ^{[1
,2
]}

机构：

[1] Univ Autonoma Barcelona, Fac Vet, Dept Sanit & Anat Anim, Cerdanyola Del Valles 08193, Spain

[2] UAB IRTA, Ctr Recerca Sanit Anim CReSA, Edifici CR, Cerdanyola Del Valles 08193, Spain

[3] Natl Inst Anim Hlth, NARO, Tsukuba, Ibaraki 305, Japan

来源：

VETERINARY RESEARCH | 2011年 / 42卷

关键词：

DENDRITIC CELLS; ALVEOLAR MACROPHAGES; INTERFERON-ALPHA; SWINE; INFECTION; SUSCEPTIBILITY; ACTIVATION; VITRO;

D O I：

10.1186/1297-9716-42-9

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-alpha. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-alpha were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.

引用

页数：10

共 32 条

[1] Innate immune responses to replication of porcine reproductive and respiratory syndrome virus in isolated swine alveolar macrophages [J].

Ait-Ali, Tahar ;

Wilson, Alison D. ;

Westcott, David G. ;

Clapperton, Mary ;

Waterfall, Martin ;

Mellencamp, Martha A. ;

Drew, Trevor W. ;

Bishop, Stephen C. ;

Archibald, Alan L. .

VIRAL IMMUNOLOGY, 2007, 20 (01) :105-118

[2] Interferon-α response to swine arterivirus (PoAV), the porcine reproductive and respiratory syndrome virus [J].

Albina, E ;

Carrat, C ;

Charley, B .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1998, 18 (07) :485-490

[3] CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses [J].

Calvert, Jay G. ;

Slade, David E. ;

Shields, Shelly L. ;

Jolie, Rika ;

Mannan, Ramasamy M. ;

Ankenbauer, Robert G. ;

Welch, Siao-Kun W. .

JOURNAL OF VIROLOGY, 2007, 81 (14) :7371-7379

[4] Porcine dendritic cells generated in vitro:: morphological, phenotypic and functional properties [J].

Carrasco, CP ;

Rigden, RC ;

Schaffner, R ;

Gerber, H ;

Neuhaus, V ;

Inumaru, S ;

Takamatsu, H ;

Bertoni, G ;

McCullough, KC ;

Summerfield, A .

IMMUNOLOGY, 2001, 104 (02) :175-184

[5] Phenotypic and functional modulation of bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus [J].

Chang, Hsueh-Chen ;

Peng, Yu-Tang ;

Chang, Hsiu-luan ;

Chaung, Hso-Chi ;

Chung, Wen-Bin .

VETERINARY MICROBIOLOGY, 2008, 129 (3-4) :281-293

[6] Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production [J].

Charerntantanakul, Wasin ;

Platt, Ratree ;

Roth, James A. .

VIRAL IMMUNOLOGY, 2006, 19 (04) :646-661

[7] Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist [J].

Chen, Z. ;

Lawson, S. ;

Sun, Z. ;

Zhou, X. ;

Guan, X. ;

Christopher-Hennings, J. ;

Nelson, E. A. ;

Fang, Y. .

VIROLOGY, 2010, 398 (01) :87-97

[8] Effects of porcine reproductive and respiratory syndrome virus (isolate tw91) on porcine alveolar macrophages in vitro [J].

Chiou, MT ;

Jeng, CR ;

Chueh, LL ;

Cheng, CH ;

Pang, VF .

VETERINARY MICROBIOLOGY, 2000, 71 (1-2) :9-25

[9] Different European-type vaccines against porcine reproductive and respiratory syndrome virus have different immunological properties and confer different protection to pigs [J].

Diaz, I. ;

Darwich, L. ;

Pappaterra, G. ;

Pujols, J. ;

Mateu, E. .

VIROLOGY, 2006, 351 (02) :249-259

[10] Immune responses of pigs after experimental infection with a European strain of Porcine reproductive and respiratory syndrome virus [J].

Díaz, I ;

Darwich, L ;

Pappaterra, G ;

Pujols, J ;

Mateu, E .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :1943-1951

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