An investigation on the distribution of Flavobacterium psychrophilum in the Umikawa River

被引:41
作者
Amita, K
Hoshino, M
Honma, T
Wakabayashi, H
机构
[1] Niigata Prefectural Inland Water Fisheries Expt S, Nagaoka, Niigata 9401137, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Tokyo 1138657, Japan
来源
FISH PATHOLOGY | 2000年 / 35卷 / 04期
关键词
Flavobacterium psychrophilum; coldwater disease; Plecoglossus altivelis; IFAT; PCR; carrier; field survey;
D O I
10.3147/jsfp.35.193
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
An investigation on the distribution of Flavobacterium psychrophilum was conducted at the Umikawa River in Niigata Prefecture in 1999. Wild ayu (Plecoglossus altivelis), released ayu and other feral fishes (Oncorhynchus masou, Trybolodon hakonensis, Cottus kazika, Chaenogobius urotaenia, Rhinogobius brunneus, Tridentiger obscurus) were caught every month from May to December. In November newly-hatched ayu were caught in the river. In addition to fish, we collected water samples and small algae growing on the surface of stones on the river bed, which ayu lived on. By nested PCR and/or IFAT, the bacterium was detected in the gills and/or kidney tissue samples of both wild and released ayu as well as of four (Oncorhynchus masou, Trybolodon hakonensis, Chaenogobius urotaenia, Rhinogobius brunneus) of the six other fish species. The prevalence of the carrier fish was generally high in June and October. The eggs taken from wild mature ayu and the larvae collected in the river were revealed also to be carriers. Algae collected in May, November acid December, and a water sample in December were found to be positive for F. psychrophilum by PCR. These findings suggest that non-ayu fishes as well as released ayu may serve as sources of infection for wild young ayu which migrate from sea to the river. It is also suggested that the coldwater disease may be transmitted from spawners to larvae.
引用
收藏
页码:193 / 197
页数:5
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