The Phenylquinazoline Compound S-4893 is a Non-Competitive Cytokinin Antagonist that Targets Arabidopsis Cytokinin Receptor CRE1 and Promotes Root Growth in Arabidopsis and Rice

被引:28
作者
Arata, Yuto [1 ]
Nagasawa-Iida, Asako [1 ]
Uneme, Hideki [1 ]
Nakajima, Hiroki [1 ]
Kakimoto, Tatsuo [2 ]
Sato, Ryo [3 ]
机构
[1] Sumitomo Chem Co Ltd, Agr Chem Res Lab, Takarazuka, Hyogo 6658555, Japan
[2] Osaka Univ, Grad Sch Sci, Dept Biol, Osaka 5600043, Japan
[3] Sumitomo Chem Co Ltd, Agr Chem Sector, Off Planning & Coordinat, Chuo Ku, Tokyo 1048260, Japan
关键词
Arabidopsis thaliana; Cytokinin; Cytokinin antagonist; Cytokinin receptor; Plant growth regulator; Root growth promoter; SIGNAL-TRANSDUCTION; ATP/ADP ISOPENTENYLTRANSFERASES; HISTIDINE KINASE; BINDING ASSAY; PLANT-GROWTH; BIOSYNTHESIS; PERCEPTION; MERISTEM; SHOOT; IDENTIFICATION;
D O I
10.1093/pcp/pcq163
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We identified two phenylquinazoline compounds in a large-scale screening for cytokinin antagonists in yeast expressing the Arabidopsis cytokinin receptor cytokinin response 1/histidine kinase 4 (CRE1). After chemical modifications, we obtained compound S-4893, which non-competitively inhibited binding of the natural ligand 2-isopentenyladenine to CRE1. S-4893 antagonized cytokinin-induced activation of the Arabidopsis response regulator 5 promoter in Arabidopsis. Importantly, S-4893 had no detectable intrinsic cytokinin agonist activity in Arabidopsis or in the transformed yeast system. Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S-4893 also promoted seminal, crown and lateral root growth in rice, suggesting that S-4893 could potentially promote root growth in a variety of agronomically important plants. We believe S-4893 will be a useful tool in functional studies of cytokinin action in a wide range of plants and a lead compound for the development of useful root growth promoters in agriculture.
引用
收藏
页码:2047 / 2059
页数:13
相关论文
共 51 条
[1]   NEW PLANT BINARY VECTORS WITH SELECTABLE MARKERS LOCATED PROXIMAL TO THE LEFT T-DNA BORDER [J].
BECKER, D ;
KEMPER, E ;
SCHELL, J ;
MASTERSON, R .
PLANT MOLECULAR BIOLOGY, 1992, 20 (06) :1195-1197
[2]   Hormone interactions at the root apical meristem [J].
Benkova, Eva ;
Hejatko, Jan .
PLANT MOLECULAR BIOLOGY, 2009, 69 (04) :383-396
[3]   Cytokinin: Perception, signal transduction, and role in plant growth and development [J].
Choi, Jaemyung ;
Hwang, Ildoo .
JOURNAL OF PLANT BIOLOGY, 2007, 50 (02) :98-108
[4]   Characterization of the response of the arabidopsis response regulator gene family to cytokinin [J].
D'Agostino, IB ;
Deruère, J ;
Kieber, JJ .
PLANT PHYSIOLOGY, 2000, 124 (04) :1706-1717
[5]   Cytokinins determine Arabidopsis root-meristem size by controlling cell differentiation [J].
Dello Ioio, Raffaele ;
Linhares, Francisco Scaglia ;
Scacchi, Emanuele ;
Casamitjana-Martinez, Eva ;
Heidstra, Renze ;
Costantino, Paolo ;
Sabatini, Sabrina .
CURRENT BIOLOGY, 2007, 17 (08) :678-682
[6]   Biosynthesis, signal transduction and molecular regulation of cytokinins [J].
Ding, Yu ;
Ma, Qing-Hu .
CURRENT PHARMACEUTICAL ANALYSIS, 2006, 2 (02) :169-182
[7]  
Dixon M., 1979, ENZYMES
[8]   The two-component signal system in rice (Oryza sativa L.):: A genome-wide study of cytokinin signal perception and transduction [J].
Du, Liming ;
Jiao, Fangchan ;
Chu, Jun ;
Jin, Gulei ;
Chen, Ming ;
Wu, Ping .
GENOMICS, 2007, 89 (06) :697-707
[9]  
Ferguson L, 2006, AGRICULTURAL MEDICINE: PRACTICE GUIDE, P156, DOI 10.1007/0-387-30105-4_15
[10]   Cytokinin signaling [J].
Ferreira, FJ ;
Kieber, JJ .
CURRENT OPINION IN PLANT BIOLOGY, 2005, 8 (05) :518-525