Typing of varicella zoster virus by amplification of DNA polymorphisms

被引：35

作者：

Hawrami, K

Harper, D

Breuer, J

机构：

[1] LONDON HOSP, COLL MED, DEPT MED MICROBIOL, LONDON E1 1BB, ENGLAND

[2] UNIV LONDON ST BARTHOLOMEWS HOSP & MED COLL, DEPT VIROL, LONDON EC1, ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1996年 / 57卷 / 02期

关键词：

PCR; VZV; typing; repeats;

D O I：

10.1016/0166-0934(95)01981-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction was used to amplify five variable regions of varicella tester virus DNA from 20 samples of vesicle fluid. Two of the regions, R1 and R5, were found to be polymorphic, with the former having three alleles (A, B and C) and the latter, two (A and B). The R1 and R5 polymorphisms were stable up to passage five in tissue culture. The sensitivity of the PCR (down to six copies) enabled detection of virus from vesicle fluid dried on glass slides and overall the method was five times more sensitive than conventional tissue culture. The method described is simple, sensitive and informative and provides a means by which questions about the epidemiology and clinical biology of VZV infection may begin to be addressed.

引用

页码：169 / 174

页数：6

共 17 条

[1] RESTRICTION FRAGMENT DIFFERENCES BETWEEN THE GENOMES OF THE OKA VARICELLA VACCINE VIRUS AND AMERICAN WILD-TYPE VARICELLA-ZOSTER VIRUS [J].