Determination of the formal reduction potential of Lumbricus terrestris hemoglobin using thin layer spectroelectrochemistry

被引：17

作者：

Dorman, SC ^{[1
]}

Harrington, JP

Martin, MS

Johnson, TV

机构：

[1] Birmingham So Coll, Dept Chem, Birmingham, AL 35254 USA

[2] SUNY Albany, Dept Chem, New Paltz, NY 12561 USA

[3] Univ S Alabama, Dept Chem, Mobile, AL 36688 USA

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2004年 / 98卷 / 01期

关键词：

Lumbricus terrestris; hemoglobins; spectroelectrochemistry; redox potentials; mediator titrants;

D O I：

10.1016/j.jinorgbio.2003.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The formal reduction potential (E-o') of Lumbricus terrestris hemoglobin was determined using thin layer spectroelectrochemistry as 0.073 (+/-0.005) V vs Ag/AgCl (0.281 V vs SHE, standard hydrogen electrode). Nernst plots of Lumbricus terrestris hemoglobin with tris-bipyridinecobalt(II) as a mediator titrant have similar linear slopes as Nernst plots of horse heart myoglobin with hexaamineruthenium(II) as a mediator titrant. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：185 / 188

页数：4

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