The role of surface basic amino acids of dengue virus NS3 helicase in viral RNA replication and enzyme activities

被引：13

作者：

Chiang, Pao-Yin ^{[1
,2
]}

Wu, Huey-Nan ^{[2
]}

机构：

[1] Natl Def Med Ctr, Grad Inst Life Sci, Taipei, Taiwan

[2] Acad Sinica, Inst Mol Biol, Taipei, Taiwan

来源：

FEBS LETTERS | 2016年 / 590卷 / 14期

关键词：

Dengue virus NS3 protein; RTPase/ATPase/unwinding activities; viral RNA replication; TYPE-2 NONSTRUCTURAL PROTEIN-3; SINGLE-STRANDED RNA; CRYSTAL-STRUCTURE; HELICASE/NUCLEOSIDE TRIPHOSPHATASE; CATALYTIC DOMAIN; SERINE-PROTEASE; NS4B; RESOLUTION; 5'-TRIPHOSPHATASE; POLYMERASE;

D O I：

10.1002/1873-3468.12232

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Structure-based mutagenesis analysis on selected conserved surface basic residues of DENV NS3 helicase was performed using a selectable replicon and recombinant protein. We found a requirement for basic side chains of NS3 residues #225, #268, and #538 to activate viral RNA replication and ensure RNA-stimulated ATPase activity, and a critical role for R560 and R599 residues in maintaining NS3 helicase structure, linked to its biological function and catalytic activity. Three screened NS3 second-site mutations for R225A and R268A/E mutations elevated the functional RNA binding of NS3 helicase and compensated the replication defect of the original NS3 mutant replicons.

引用

页码：2307 / 2320

页数：14

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