Simultaneous prediction of human antithrombin III and main metabolites in animal cell culture processes by near-infrared spectroscopy

被引：6

作者：

Harthun, S ^{[1
]}

Matischak, K ^{[1
]}

Friedl, P ^{[1
]}

机构：

[1] Tech Univ Darmstadt, Inst Biochem, D-64287 Darmstadt, Germany

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 05期

关键词：

D O I：

10.1023/A:1008882516700

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Samples of cell culture supernatants were analysed by near-infrared (NIR) spectroscopy. Spectral information was used to generate calibration models by using multivariate partial least squares (PLS) regression. Concentration predictions by these models were in good agreement with the values produced by conventional methods. Due to the simple pre-treatment of the sample and the short time required for measurement and prediction, NIR is a promising tool for automation of a cell culture process.

引用

页码：393 / 397

页数：5

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