A liquid crystalline medium for measuring residual dipolar couplings over a wide range of temperatures

被引：72

作者：

Wang, H ^{[1
]}

Eberstadt, M ^{[1
]}

Olejniczak, ET ^{[1
]}

Meadows, RP ^{[1
]}

Fesik, SW ^{[1
]}

机构：

[1] Abbott Labs, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 1998年 / 12卷 / 03期

关键词：

bicelle; lipid; liquid crystal; protein alignment; residual dipolar coupling;

D O I：

10.1023/A:1008361931685

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mixture of dilauroyl phosphatidylcholine (DLPC) and 3-(cholamidopropyl)dimethylammonio-2-hydroxyl-1-propane sulfonate (CHAPSO) in water forms disc shaped bicelles that become ordered at high magnetic fields over a wide range of temperatures. As illustrated for the FK506 binding protein (FKBP), large residual dipolar couplings can be measured for proteins dissolved in low concentrations (5% w/v) of a DLPC/CHAPSO medium at a molar ratio of 4.2:1. This system is especially useful for measuring residual dipolar couplings for molecules that are only stable at low temperatures.

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收藏

页码：443 / 446

页数：4

相关论文

共 11 条

[1] High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium [J].

Bax, A ;

Tjandra, N .

JOURNAL OF BIOMOLECULAR NMR, 1997, 10 (03) :289-292

[2] Magnetic alignment of duplex and quadruplex DNAs [J].

Kung, HC ;

Wang, KY ;

Goljer, I ;

Bolton, PH .

JOURNAL OF MAGNETIC RESONANCE SERIES B, 1995, 109 (03) :323-325

[3] MAGNETICALLY-ORIENTED PHOSPHOLIPID MICELLES AS A TOOL FOR THE STUDY OF MEMBRANE-ASSOCIATED MOLECULES [J].

SANDERS, CR ;

HARE, BJ ;

HOWARD, KP ;

PRESTEGARD, JH .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 1994, 26 (pt 5) :421-444

[4] CHARACTERIZATION OF MAGNETICALLY ORIENTABLE BILAYERS IN MIXTURES OF DIHEXANOYLPHOSPHATIDYLCHOLINE AND DIMYRISTOYLPHOSPHATIDYLCHOLINE BY SOLID-STATE NMR [J].

SANDERS, CR ;

SCHWONEK, JP .

BIOCHEMISTRY, 1992, 31 (37) :8898-8905

[5] MAGNETIC-ORDERING OF PHOSPHOLIPID-MEMBRANES [J].

SEELIG, J ;

BORLE, F ;

CROSS, TA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 814 (01) :195-198

[6] Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium [J].

Tjandra, N ;

Bax, A .

SCIENCE, 1997, 278 (5340) :1111-1114

[7] Measurement of dipolar contributions to (1)J(CH) splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra [J].

Tjandra, N ;

Bax, A .

JOURNAL OF MAGNETIC RESONANCE, 1997, 124 (02) :512-515

[8] Use of dipolar H-1-N-15 and H-1-C-13 couplings in the structure determination of magnetically oriented macromolecules in solution [J].

Tjandra, N ;

Omichinski, JG ;

Gronenborn, AM ;

Clore, GM ;

Bax, A .

NATURE STRUCTURAL BIOLOGY, 1997, 4 (09) :732-738

[9]

TJANDRA N, 1996, J AM CHEM SOC, V118, P6244

[10] NUCLEAR MAGNETIC DIPOLE INTERACTIONS IN FIELD-ORIENTED PROTEINS - INFORMATION FOR STRUCTURE DETERMINATION IN SOLUTION [J].

TOLMAN, JR ;

FLANAGAN, JM ;

KENNEDY, MA ;

PRESTEGARD, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9279-9283