Correlations between molecular numbers and molecular masses in an evanescent field and their applications in probing molecular interactions

被引：18

作者：

Gai, HW

Wang, Q

Ma, YF

Lin, BC

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

[2] Univ Missouri, Dept Chem, Rolla, MO 65409 USA

[3] Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 32期

关键词：

evanescent field; fluorescence; molecule counting; oligonucleotides; single-molecule studies;

D O I：

10.1002/anie.200500461

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Complex numbers: The number of molecules detected in the evanescent field during single-molecule fluorescence imaging decreases with increasing molecular weight in free solution (see graphic; FITC = fluorescein isothiocyanate). This technique could be useful for screening undefined molecular interactions at extremely low concentrations without separation. (Graph Presented) © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：5107 / 5110

页数：4

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