Novel cationic copolymers of a polyasparthylhydrazide: Synthesis and characterization

被引：11

作者：

Cavallaro, G ^{[1
]}

Palumbo, FS ^{[1
]}

Licciardi, M ^{[1
]}

Giammona, G ^{[1
]}

机构：

[1] Univ Palermo, Dipartimento Chim & Tecnol Farmaceut, I-90123 Palermo, Italy

来源：

DRUG DELIVERY | 2005年 / 12卷 / 06期

关键词：

gene delivery; interpolyelectrolytes; polyaspartylhydrazide; polycations; 3-(carboxypropyl)trimethyl ammonium chloride;

D O I：

10.1080/10717540590968792

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

alpha,beta-poly(asparthylhydrazide) (PAHy), a water soluble synthetic polymer, was functionalized by using EDCI chemistry with 3-(carboxypropyl) trimethyl-ammonium chloride (CPTACl) obtaining carboxypropyltrimethyl ammonium copolymers (PAHy-CPTA). Three PAHy-CPTA copolymers at increasing derivatization degrees (38%, 48%, 58%) were chosen for subsequent investigations. The capability of these copolymers to bind, neutralize, and protect DNA against degradation by DNase II was evalued by gel retardation assay and DNA degradation test at pH 5.5. Zeta potential measurements show that all studied polymers are able to neutralize the anionic charge of DNA at polymer/DNA weight ratio in the range of 0.8/1-5/1. Polyplex dimensional distribution analyses in bistilled water, saline solution NaCl 0.9%, and HEPES pH 7 show that polyplex size is strongly affected by both presence and type of electrolyte and with time incubation.

引用

页码：377 / 384

页数：8

共 20 条

[1]

A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: Polyethylenimine [J].