Regulation of the leucoanthocyanidin dioxygenase gene expression in Vitis vinifera

被引：104

作者：

Gollop, R ^{[1
]}

Farhi, S ^{[1
]}

Perl, A ^{[1
]}

机构：

[1] Agr Res Org, Volcani Ctr, Dept Fruit Tree Breeding & Mol Genet, IL-50250 Bet Dagan, Israel

来源：

PLANT SCIENCE | 2001年 / 161卷 / 03期

关键词：

anthocyanins; gene expression; grape; ldox; light signaling; sucrose signaling; promoter;

D O I：

10.1016/S0168-9452(01)00445-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The control of expression of the leucoanthocyanidin dioxygenase (LDOX) gene was studied in grape. LDOX converts leucoanthocyanidins to anthocyanidins in the anthocyanins pathway. In the present study, the promoter of the Idox gene was cloned and fused to the uidA reporter gene, and the expression of this fusion was analyzed in transformed plants and in transformed red fruit cell suspensions. The Idox promoter-uidA gene fusion was expressed in all the plant organs. White light, calcium and sucrose induced the ldox gene expression. The induction by both light and calcium under light suggests the possible involvement of a UV receptors signal transduction pathway in the induction of the ldox gene. The induction of the Idox gene by light and by sucrose under light indicates a close interaction between the sucrose and the light signaling pathways. Analysis of the Idox promoter sequence revealed the existence of several putative DNA binding motifs. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：579 / 588

页数：10

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