Rapid analysis of the Vβ repertoire of CD4 and CD8 T lymphocytes in whole blood

被引：15

作者：

Maclsaac, C

Curtis, N

Cade, J

Visvanathan, K

机构：

[1] Murdoch Childrens Res Inst, Parkville, Vic 3052, Australia

[2] Royal Melbourne Hosp, Intens Care Unit, Melbourne, Vic, Australia

[3] Royal Childrens Hosp, Dept Gen Med, Melbourne, Vic, Australia

[4] Univ Melbourne, Dept Paediat, Melbourne, Vic, Australia

[5] Univ Melbourne, Royal Melbourne Hosp, Dept Med, Melbourne, Vic 3050, Australia

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2003年 / 283卷 / 1-2期

关键词：

T-cell receptor; superantigens; flow cytometry; lymphocytes; toxic shock; genes; T-cell receptor beta;

D O I：

10.1016/S0022-1759(03)00260-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determination of the T cell receptor Vbeta repertoire of human CD4 and CD8 populations is a useful immunological tool, particularly in the investigation of superantigen involvement in various disease states. We describe the optimisation of a rapid technique for the simultaneous evaluation of 24 Vbeta families of the T cell receptor of CD4 and CD8 positive lymphocytes in whole blood by flow cytometry adapting a commercially available monoclonal antibody kit. The technique described is reliable and reproducible, and we describe its use as a potential diagnostic tool in patients with staphylococcal and streptococcal toxic shock syndromes. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：9 / 15

页数：7

共 26 条

[1] Structure and function of streptococcal and staphylococcal superantigens in septic shock [J].