Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling

被引:395
作者
Yoo, Sang-Dong [1 ,2 ]
Cho, Young-Hee [1 ,2 ]
Tena, Guillaume [1 ,2 ]
Xiong, Yan [1 ,2 ]
Sheen, Jen [1 ,2 ]
机构
[1] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
关键词
D O I
10.1038/nature06543
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A principal question in MAP kinase ( MAPK/ MPK) cascade signalling is how similar components dictate different specificity in the information- processing machineries from yeast to humans and plants. In Arabidopsis, how MPK3/ 6modulates distinct outputs in diverse signal transduction pathways remains elusive. By combining systematic cellular and genetic screens, here we uncover a previously unexpected MKK9 - MPK3/ MPK6 cascade promoting ethylene- insensitive 3 ( EIN3)- mediated transcription in ethylene signalling. The mkk9 mutant exhibits a broad spectrum of moderate ethylene- insensitive phenotypes, and translocated MKK9 governs nuclear signalling downstream of receptors. Breaking a linear model and conventional MAPK signalling, ethylene inactivates the negative regulator constitutive triple response 1 ( CTR1, a Raf- like MAPK kinase kinase ( MAPKKK)) to activate the positive MKK9 - MPK3/ 6 cascade. The bifurcate and antagonistic CTR1 and MKK9 pathways are both critical in determining ethylene- signalling specificity through two MAPK phosphorylation sites with opposite effects on EIN3 stability. The results suggest a new paradigm for linking intertwined MAPK cascades to control quantitative responses and specificity in signalling networks.
引用
收藏
页码:789 / U1
页数:8
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[1]   Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function [J].
Achard, P ;
Vriezen, WH ;
Van Der Straeten, D ;
Harberd, NP .
PLANT CELL, 2003, 15 (12) :2816-2825
[2]   EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis [J].
Alonso, JM ;
Hirayama, T ;
Roman, G ;
Nourizadeh, S ;
Ecker, JR .
SCIENCE, 1999, 284 (5423) :2148-2152
[3]   The ethylene signaling pathway [J].
Alonso, JM ;
Stepanova, AN .
SCIENCE, 2004, 306 (5701) :1513-1515
[4]   Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].
Alonso, JM ;
Stepanova, AN ;
Leisse, TJ ;
Kim, CJ ;
Chen, HM ;
Shinn, P ;
Stevenson, DK ;
Zimmerman, J ;
Barajas, P ;
Cheuk, R ;
Gadrinab, C ;
Heller, C ;
Jeske, A ;
Koesema, E ;
Meyers, CC ;
Parker, H ;
Prednis, L ;
Ansari, Y ;
Choy, N ;
Deen, H ;
Geralt, M ;
Hazari, N ;
Hom, E ;
Karnes, M ;
Mulholland, C ;
Ndubaku, R ;
Schmidt, I ;
Guzman, P ;
Aguilar-Henonin, L ;
Schmid, M ;
Weigel, D ;
Carter, DE ;
Marchand, T ;
Risseeuw, E ;
Brogden, D ;
Zeko, A ;
Crosby, WL ;
Berry, CC ;
Ecker, JR .
SCIENCE, 2003, 301 (5633) :653-657
[5]   Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis [J].
Alonso, JM ;
Stepanova, AN ;
Solano, R ;
Wisman, E ;
Ferrari, S ;
Ausubel, FM ;
Ecker, JR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2992-2997
[6]   MAP kinase signalling cascade in Arabidopsis innate immunity [J].
Asai, T ;
Tena, G ;
Plotnikova, J ;
Willmann, MR ;
Chiu, WL ;
Gomez-Gomez, L ;
Boller, T ;
Ausubel, FM ;
Sheen, J .
NATURE, 2002, 415 (6875) :977-983
[7]   The Arabidopsis EIN3 binding F-box proteins EBF1 and EBF2 have distinct but overlapping roles in ethylene signaling [J].
Binder, Brad M. ;
Walker, Joseph M. ;
Gagne, Jennifer M. ;
Emborg, Thomas J. ;
Hemmann, Georg ;
Bleecker, Anthony B. ;
Vierstra, Richard D. .
PLANT CELL, 2007, 19 (02) :509-523
[8]   Ethylene: A gaseous signal molecule in plants [J].
Bleecker, AB ;
Kende, H .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :1-+
[9]   Efficient virus-induced gene silencing in Arabidopsis [J].
Burch-Smith, Tessa M. ;
Schiff, Michael ;
Liu, Yule ;
Dinesh-Kumar, S. P. .
PLANT PHYSIOLOGY, 2006, 142 (01) :21-27
[10]   Modulation of ethylene responses affects plant salt-stress responses [J].
Cao, Wan-Hong ;
Liu, Jun ;
He, Xin-Jian ;
Mu, Rui-Ling ;
Zhou, Hua-Lin ;
Chen, Shou-Yi ;
Zhang, Jin-Song .
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